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Two-photon fluctuation correlation s...

Berland, Keith Michael.

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Two-photon fluctuation correlation spectroscopy: Method and applications to protein aggregation and intracellular diffusion.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Two-photon fluctuation correlation spectroscopy: Method and applications to protein aggregation and intracellular diffusion./
Author:	Berland, Keith Michael.
Description:	92 p.
Notes:	Source: Dissertation Abstracts International, Volume: 57-04, Section: B, page: 2411.
Contained By:	Dissertation Abstracts International57-04B.
Subject:	Biophysics, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9624286

Two-photon fluctuation correlation spectroscopy: Method and applications to protein aggregation and intracellular diffusion.
Berland, Keith Michael.

Two-photon fluctuation correlation spectroscopy: Method and applications to protein aggregation and intracellular diffusion. - 92 p.

Source: Dissertation Abstracts International, Volume: 57-04, Section: B, page: 2411.

Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1995.

Fluctuation correlation spectroscopy (FCS) is an experimental technique for the study of hydrodynamics and molecular interactions of microscopic systems. The method monitors the statistical behavior of spontaneous equilibrium fluctuations in number occupation of a fluorescent species in a subvolume of a sample. Microscopic models have been developed which relate observed statistical fluctuations in fluorescence observables to physical quantities of interest. This thesis presents the first application of two-photon molecular excitation to FCS. Two-photon excitation offers several advantages over one-photon excitation for FCS experiments. These advantages are discussed, and model systems are studied to calibrate and characterize the capabilities of the technique. The theoretical framework necessary to recover physical parameters from observed fluctuation spectra is also presented.Subjects--Topical Terms:

1019105
Biophysics, General.

Two-photon fluctuation correlation spectroscopy: Method and applications to protein aggregation and intracellular diffusion.
LDR:02422nmm 2200277 4500 001 1815805
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008 130610s1995 eng d
035 $a (UnM)AAI9624286
035 $a AAI9624286
040 $a UnM $c UnM
100 1 $a Berland, Keith Michael. $3 1905212
245 1 0 $a Two-photon fluctuation correlation spectroscopy: Method and applications to protein aggregation and intracellular diffusion.
300 $a 92 p.
500 $a Source: Dissertation Abstracts International, Volume: 57-04, Section: B, page: 2411.
500 $a Adviser: E. Gratton.
502 $a Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1995.
520 $a Fluctuation correlation spectroscopy (FCS) is an experimental technique for the study of hydrodynamics and molecular interactions of microscopic systems. The method monitors the statistical behavior of spontaneous equilibrium fluctuations in number occupation of a fluorescent species in a subvolume of a sample. Microscopic models have been developed which relate observed statistical fluctuations in fluorescence observables to physical quantities of interest. This thesis presents the first application of two-photon molecular excitation to FCS. Two-photon excitation offers several advantages over one-photon excitation for FCS experiments. These advantages are discussed, and model systems are studied to calibrate and characterize the capabilities of the technique. The theoretical framework necessary to recover physical parameters from observed fluctuation spectra is also presented.
520 $a There are two biophysical applications proposed and discussed in this thesis. The first is the study of particle mobilities and intracellular diffusion. Measurements of particle diffusion in the cytoplasm of live cells are presented. The second application is the detection of protein aggregation in solution using two-photon scanning FCS. Measurements are shown demonstrating the potential to study aggregation equilibria at low protein concentrations, and the dissociation reactions of several oligomeric proteins are studied. The possibility to measure dissociation kinetics is also demonstrated.
590 $a School code: 0090.
650 4 $a Biophysics, General. $3 1019105
650 4 $a Physics, Optics. $3 1018756
690 $a 0786
690 $a 0752
710 2 0 $a University of Illinois at Urbana-Champaign. $3 626646
773 0 $t Dissertation Abstracts International $g 57-04B.
790 1 0 $a Gratton, E., $e advisor
790 $a 0090
791 $a Ph.D.
792 $a 1995
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9624286