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Development of HPLC and mass spectro...

Wang, Sheng.

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Development of HPLC and mass spectrometric methods for determination and separation of cytokines.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Development of HPLC and mass spectrometric methods for determination and separation of cytokines./
Author:	Wang, Sheng.
Description:	80 p.
Notes:	Source: Masters Abstracts International, Volume: 44-01, page: 0349.
Contained By:	Masters Abstracts International44-01.
Subject:	Chemistry, Analytical. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1428338
ISBN:	0542256800

Development of HPLC and mass spectrometric methods for determination and separation of cytokines.
Wang, Sheng.

Development of HPLC and mass spectrometric methods for determination and separation of cytokines. - 80 p.

Source: Masters Abstracts International, Volume: 44-01, page: 0349.

Thesis (M.S.)--The University of North Carolina at Greensboro, 2005.

A great deal of success has been achieved in the understanding of cytokine structures, functions, and synthesis. However, the understanding of the sophisticated cytokine network and how it is regulated physiologically is still limited. In order to improve this understanding, the development of rapid and convenient methods to separate and characterize the cytokines is necessary. The goal of this research is to establish such a method using high performance liquid chromatography (HPLC) coupled to electrospray ionization mass spectrometry (ESI-MS).

ISBN: 0542256800Subjects--Topical Terms:

586156
Chemistry, Analytical.

Development of HPLC and mass spectrometric methods for determination and separation of cytokines.
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020 $a 0542256800
035 $a (UnM)AAI1428338
035 $a AAI1428338
040 $a UnM $c UnM
100 1 $a Wang, Sheng. $3 1902677
245 1 0 $a Development of HPLC and mass spectrometric methods for determination and separation of cytokines.
300 $a 80 p.
500 $a Source: Masters Abstracts International, Volume: 44-01, page: 0349.
500 $a Director: Nadja B. Cech.
502 $a Thesis (M.S.)--The University of North Carolina at Greensboro, 2005.
520 $a A great deal of success has been achieved in the understanding of cytokine structures, functions, and synthesis. However, the understanding of the sophisticated cytokine network and how it is regulated physiologically is still limited. In order to improve this understanding, the development of rapid and convenient methods to separate and characterize the cytokines is necessary. The goal of this research is to establish such a method using high performance liquid chromatography (HPLC) coupled to electrospray ionization mass spectrometry (ESI-MS).
520 $a In the research described here, first, conventional electrospray mass spectrometry and nanospray ionization mass spectrometry methods were compared for the analysis of various small proteins, including several cytokines. The detection limits achieved for the various proteins using nanospray ionization were several orders of magnitude lower than those achieved with conventional electrospray ionization mass spectrometry techniques. Second, the use of a heparin column for concentration of proteins prior to analysis was evaluated. Finally, separation methods for different cytokines using capillary HPLC coupled to nanospray ionization mass spectrometry were developed. Separation of the cytokines TNF-alpha and IL-1 was achieved using this technique with a resolution of approximately 3.
590 $a School code: 0154.
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0486
690 $a 0487
710 2 0 $a The University of North Carolina at Greensboro. $3 1029687
773 0 $t Masters Abstracts International $g 44-01.
790 1 0 $a Cech, Nadja B., $e advisor
790 $a 0154
791 $a M.S.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1428338