東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Lipid-based oxidative protein modifi...

Suresh Babu, Annangudi Palani.

Linked to FindBook

Google Book

Amazon

博客來

Lipid-based oxidative protein modifications in glaucoma.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Lipid-based oxidative protein modifications in glaucoma./
Author:	Suresh Babu, Annangudi Palani.
Description:	301 p.
Notes:	Source: Dissertation Abstracts International, Volume: 66-10, Section: B, page: 5332.
Contained By:	Dissertation Abstracts International66-10B.
Subject:	Health Sciences, Ophthalmology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3193534
ISBN:	0542369338

Lipid-based oxidative protein modifications in glaucoma.
Suresh Babu, Annangudi Palani.

Lipid-based oxidative protein modifications in glaucoma. - 301 p.

Source: Dissertation Abstracts International, Volume: 66-10, Section: B, page: 5332.

Thesis (Ph.D.)--Case Western Reserve University, 2006.

We and others have postulated that oxidative protein modifications, including covalent crosslinks, may accumulate in trabecular meshwork (TM) and contribute to impaired aqueous outflow and primary open angle glaucoma (POAG). To test this hypothesis, human TM from normal and POAG donors was probed with antibodies for oxidative protein modifications. Studies using SDS PAGE and Western blotting showed elevated levels of covalent protein modifications derived from lipid oxidation products (iso[4]Levuglandin E2 (isoLGE 2) and 4-hydroxy-nonenal (HNE)), an advanced glycation end product (methylglyoxal), and a tryptophan oxidation product (3-hydroxykynurenine), in POAG compared to age-and-gender matched controls. Immunoprecipitation (IP) using iso[4]LGE 2 and HNE antibodies showed the presence of several apparently crosslinked proteins in POAG donor TM. 2D PAGE Western analyses, also showed proteins with altered molecular weights and pIs, implying modified and/or crosslinked proteins. Immunohistochemistry showed that iso[4]LGE2 and HNE modifications were localized to the TM. Thus, the study provides direct evidence for lipid-based oxidative modification of TM proteins in POAG. Additionally, increased levels of iso[4]LGE2 in the TM of the DBA/2J mouse model of glaucoma was also established and several putative crosslinked proteins were identified using IP and mass spectrometry. These results support a role for levuglandins (LGs) in POAG pathology. LGs are known to avidly bind with and crosslink proteins.

ISBN: 0542369338Subjects--Topical Terms:

1019445
Health Sciences, Ophthalmology.

Lipid-based oxidative protein modifications in glaucoma.
LDR:03758nmm 2200361 4500 001 1814299
005 20060511141249.5
008 130610s2006 eng d
020 $a 0542369338
035 $a (UnM)AAI3193534
035 $a AAI3193534
040 $a UnM $c UnM
100 1 $a Suresh Babu, Annangudi Palani. $3 1903772
245 1 0 $a Lipid-based oxidative protein modifications in glaucoma.
300 $a 301 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-10, Section: B, page: 5332.
500 $a Advisers: Robert Gerd Salomon; John W. Crabb; Sanjoy K. Bhattacharya.
502 $a Thesis (Ph.D.)--Case Western Reserve University, 2006.
520 $a We and others have postulated that oxidative protein modifications, including covalent crosslinks, may accumulate in trabecular meshwork (TM) and contribute to impaired aqueous outflow and primary open angle glaucoma (POAG). To test this hypothesis, human TM from normal and POAG donors was probed with antibodies for oxidative protein modifications. Studies using SDS PAGE and Western blotting showed elevated levels of covalent protein modifications derived from lipid oxidation products (iso[4]Levuglandin E2 (isoLGE 2) and 4-hydroxy-nonenal (HNE)), an advanced glycation end product (methylglyoxal), and a tryptophan oxidation product (3-hydroxykynurenine), in POAG compared to age-and-gender matched controls. Immunoprecipitation (IP) using iso[4]LGE 2 and HNE antibodies showed the presence of several apparently crosslinked proteins in POAG donor TM. 2D PAGE Western analyses, also showed proteins with altered molecular weights and pIs, implying modified and/or crosslinked proteins. Immunohistochemistry showed that iso[4]LGE2 and HNE modifications were localized to the TM. Thus, the study provides direct evidence for lipid-based oxidative modification of TM proteins in POAG. Additionally, increased levels of iso[4]LGE2 in the TM of the DBA/2J mouse model of glaucoma was also established and several putative crosslinked proteins were identified using IP and mass spectrometry. These results support a role for levuglandins (LGs) in POAG pathology. LGs are known to avidly bind with and crosslink proteins.
520 $a The inability of macrophages to processes modified proteins contributes to "foam cell" formation and atherosclerosis (AS). To identify the modified proteins in inflamed macrophages, lipopolysaccharide (LPS) stimulated macrophages were studied. LPS stimulation increased the levels of LGE2 modified proteins in macrophages. The modified proteins were identified by 2D PAGE and mass spectrometry. Many of the modified proteins are involved in cholesterol trafficking, gene expression, and/or lipid efflux. This suggests a role for LGE2 in AS. These observations inspired a pilot study to explore the possible role of LGs in LPS-induced inflammation of cornea in vivo. We found that LPS promotes the generation of LGs in cornea.
520 $a An efficient synthesis of the gamma-keto-alpha,beta-unsaturated alkenoic acid functional array present in some of the oxidatively truncated phospholipids, was achieved by oxidation of furyl precursors using NaClO 2. Additionally, detection and structural characterization of multiple HNE adducts onto lysine and histidine side-chain residues were accomplished using deuterated HNE and mass spectrometry.
590 $a School code: 0042.
650 4 $a Health Sciences, Ophthalmology. $3 1019445
650 4 $a Health Sciences, Toxicology. $3 1017752
650 4 $a Chemistry, Analytical. $3 586156
650 4 $a Chemistry, Organic. $3 516206
650 4 $a Health Sciences, Pathology. $3 1017854
690 $a 0381
690 $a 0383
690 $a 0486
690 $a 0490
690 $a 0571
710 2 0 $a Case Western Reserve University. $3 1017714
773 0 $t Dissertation Abstracts International $g 66-10B.
790 1 0 $a Salomon, Robert Gerd, $e advisor
790 1 0 $a Crabb, John W., $e advisor
790 1 0 $a Bhattacharya, Sanjoy K., $e advisor
790 $a 0042
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3193534