東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Functional characterization of the t...

Pao, Gerald M.

FindBook

Google Book

Amazon

博客來

Functional characterization of the tumor suppressor BRCA1.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Functional characterization of the tumor suppressor BRCA1./
作者:	Pao, Gerald M.
面頁冊數:	282 p.
附註:	Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2426.
Contained By:	Dissertation Abstracts International66-05B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3175280
ISBN:	0542137801

Functional characterization of the tumor suppressor BRCA1.
Pao, Gerald M.

Functional characterization of the tumor suppressor BRCA1. - 282 p.

Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2426.

Thesis (Ph.D.)--University of California, San Diego, 2005.

BRCA1 is a tumor suppressor involved in Breast and ovarian cancer encoded by a 1863 amino acid protein. The strongest lead for a biological function of BRCA1 is that of a role in either DNA repair per se or in the regulation of the response to DNA damage. Other work has also implicated a role of BRCA1 in the regulation of transcription in some unknown capacity. Work in Animals have shown that BRCA1 is required for embryonic proliferation, that BRCA1 deficient cells display genomic instability and that BRCA1 deficiency leads to a severe proliferative defect. My work has attempted to elucidate the molecular functions of BRCA1. Early work in our lab showed that BRCA1 could possibly be a transcription factor. Assuming that this would be true, we set out to identify the coactivators of BRCA1. Thus we found that p300 and CBP, the histone acetyltransferases formed a complex with BRCA1 and were able to modulate the activity of BRCA1 in the transcription of artificial reporter constructs as well as the viral Moloney murine leukemia virus (MLV) and Rous Sarcoma Virus (RSV) long terminal repeat (LTR) promoters. This also prompted us to investigate if BRCA1 was a classical transcription factor in biding to these promoters. To our surprise, our work showed that BRCA1 bound DNA nonspecifically and thus we considered it unlikely that BRCA1 could be classical transcription factor and thus had to modulate the activity of promoters in some more general fashion. This led us to attempt to see whether BRCA1 was working at the chromatin level. It was in this way that we found that BRCA1 was part of the hSWI/SNF complex. We showed that the hSWI/SNF complex is required for BRCA1 mediated resistance to ionizing radiation. At the mechanistic level we found that BARD1,a binding partner for BRCA1 was able to cooperate with BRCA1 in the ubiquitin ligase activity.

ISBN: 0542137801Subjects--Topical Terms:

1017719
Biology, Molecular.

Functional characterization of the tumor suppressor BRCA1.
LDR:02743nmm 2200277 4500 001 1812935
005 20060427132645.5
008 130610s2005 eng d
020 $a 0542137801
035 $a (UnM)AAI3175280
035 $a AAI3175280
040 $a UnM $c UnM
100 1 $a Pao, Gerald M. $3 1902468
245 1 0 $a Functional characterization of the tumor suppressor BRCA1.
300 $a 282 p.
500 $a Source: Dissertation Abstracts International, Volume: 66-05, Section: B, page: 2426.
500 $a Chair: Inder M. Verma.
502 $a Thesis (Ph.D.)--University of California, San Diego, 2005.
520 $a BRCA1 is a tumor suppressor involved in Breast and ovarian cancer encoded by a 1863 amino acid protein. The strongest lead for a biological function of BRCA1 is that of a role in either DNA repair per se or in the regulation of the response to DNA damage. Other work has also implicated a role of BRCA1 in the regulation of transcription in some unknown capacity. Work in Animals have shown that BRCA1 is required for embryonic proliferation, that BRCA1 deficient cells display genomic instability and that BRCA1 deficiency leads to a severe proliferative defect. My work has attempted to elucidate the molecular functions of BRCA1. Early work in our lab showed that BRCA1 could possibly be a transcription factor. Assuming that this would be true, we set out to identify the coactivators of BRCA1. Thus we found that p300 and CBP, the histone acetyltransferases formed a complex with BRCA1 and were able to modulate the activity of BRCA1 in the transcription of artificial reporter constructs as well as the viral Moloney murine leukemia virus (MLV) and Rous Sarcoma Virus (RSV) long terminal repeat (LTR) promoters. This also prompted us to investigate if BRCA1 was a classical transcription factor in biding to these promoters. To our surprise, our work showed that BRCA1 bound DNA nonspecifically and thus we considered it unlikely that BRCA1 could be classical transcription factor and thus had to modulate the activity of promoters in some more general fashion. This led us to attempt to see whether BRCA1 was working at the chromatin level. It was in this way that we found that BRCA1 was part of the hSWI/SNF complex. We showed that the hSWI/SNF complex is required for BRCA1 mediated resistance to ionizing radiation. At the mechanistic level we found that BARD1,a binding partner for BRCA1 was able to cooperate with BRCA1 in the ubiquitin ligase activity.
590 $a School code: 0033.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Health Sciences, Oncology. $3 1018566
690 $a 0307
690 $a 0992
710 2 0 $a University of California, San Diego. $3 1018093
773 0 $t Dissertation Abstracts International $g 66-05B.
790 1 0 $a Verma, Inder M., $e advisor
790 $a 0033
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3175280