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Regulation of targeted gene repair b...

Brachman, Erin E.

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Regulation of targeted gene repair by DNA replication.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Regulation of targeted gene repair by DNA replication./
Author:	Brachman, Erin E.
Description:	184 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-12, Section: B, page: 6186.
Contained By:	Dissertation Abstracts International65-12B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3157278
ISBN:	049689708X

Regulation of targeted gene repair by DNA replication.
Brachman, Erin E.

Regulation of targeted gene repair by DNA replication. - 184 p.

Source: Dissertation Abstracts International, Volume: 65-12, Section: B, page: 6186.

Thesis (Ph.D.)--University of Delaware, 2005.

The repair of point mutations can be directed by modified single-stranded DNA oligonucleotides and regulated by cellular activities including homologous recombination, mismatch repair, and transcription. Evidence for a role of DNA replication in the regulation of gene repair is presented.

ISBN: 049689708XSubjects--Topical Terms:

1017719
Biology, Molecular.

Regulation of targeted gene repair by DNA replication.
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035 $a (UnM)AAI3157278
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040 $a UnM $c UnM
100 1 $a Brachman, Erin E. $3 1902223
245 1 0 $a Regulation of targeted gene repair by DNA replication.
300 $a 184 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-12, Section: B, page: 6186.
500 $a Professor in charge: Eric B. Kmiec.
502 $a Thesis (Ph.D.)--University of Delaware, 2005.
520 $a The repair of point mutations can be directed by modified single-stranded DNA oligonucleotides and regulated by cellular activities including homologous recombination, mismatch repair, and transcription. Evidence for a role of DNA replication in the regulation of gene repair is presented.
520 $a A Saccharomyces cerevisiae targeting assay exhibited a repair preference for certain base mismatches and a repair bias, in most cases, in favor of the nontranscribed strand. In one case, though, a higher level of correction was observed when an oligonucleotide complementary to the transcribed strand was used. Analysis of reaction intermediates provided preliminary evidence of DNA replication as a regulator of gene repair.
520 $a An SV40-virus-based system in mammalian cells then was used to investigate the role of DNA synthesis on gene repair in COS-1 cells. The influences of transcription and replication on strand bias were discriminated by changing the orientation of the eGFP relative to the origin of replication; the previously observed bias towards the nontranscribed strand was confirmed. DNA replication increases the level of nontranscribed preference only if that strand also serves as the lagging strand in DNA synthesis. The results also indicate that actively replicating molecules support oligonucleotide directed gene repair at a higher level than targets located adjacent to either slow or non-firing replication origins.
520 $a The mammalian cell line, DLD-1, containing an integrated mutant eGFP gene, was used as an assay system to explore how replication fork activity affects the overall repair reaction. The addition of 2'3 '-dideoxycytidine, a nucleoside analog that retards the rate of elongation and effectively stalls the replication fork, results in a lengthened S phase and an increased number of gene repair events. In contrast, the nucleoside analog, 1-beta-D-arabinofuranosylcytosine which stops replication in these cells, failed to stimulate the gene repair reaction to any appreciable degree until the block is released and active replication resumes. Furthermore, over-expression of wild type p53 which is known to bind transiently to stalled replication forks blocked the stimulatory effect of 2'3' -dideoxycytidine. The results indicate that an expansion of S phase and a transient stalling of replication forks can increase the frequency of targeted gene repair.
590 $a School code: 0060.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Genetics. $3 1017730
690 $a 0307
690 $a 0369
710 2 0 $a University of Delaware. $3 1017826
773 0 $t Dissertation Abstracts International $g 65-12B.
790 1 0 $a Kmiec, Eric B., $e advisor
790 $a 0060
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3157278