東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Defining the Vibrio harveyi quorum s...

Mok, Kenny Chee-Kin.

Linked to FindBook

Google Book

Amazon

博客來

Defining the Vibrio harveyi quorum sensing network.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Defining the Vibrio harveyi quorum sensing network./
Author:	Mok, Kenny Chee-Kin.
Description:	140 p.
Notes:	Source: Dissertation Abstracts International, Volume: 65-07, Section: B, page: 3312.
Contained By:	Dissertation Abstracts International65-07B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3142349
ISBN:	0496893300

Defining the Vibrio harveyi quorum sensing network.
Mok, Kenny Chee-Kin.

Defining the Vibrio harveyi quorum sensing network. - 140 p.

Source: Dissertation Abstracts International, Volume: 65-07, Section: B, page: 3312.

Thesis (Ph.D.)--Princeton University, 2004.

Vibrio harveyi regulates light production using a cell-cell communication phenomenon called quorum sensing. V. harveyi produces, detects and responds to signaling molecules called autoinducers, whose concentration is correlated with cell density. A multichannel phosphorelay circuit controls light production in response to two autoinducers, AI-1 and AI-2. This thesis shows that AI-1 and AI-2 act synergistically to regulate quorum sensing controlled genes, and that the signaling circuit in V. harveyi acts primarily as a coincidence detector to distinguish between the simultaneous presence of AI-1 and AI-2 and all other conditions (no AI, AI-1 only, or AI-2 alone). Either autoinducer alone can minimally activate or repress target gene expression; however, significant regulation only occurs in the presence of both autoinducers. I propose that a coincidence detector is used to filter out noise from the environment leading to a highly accurate quorum sensing response.

ISBN: 0496893300Subjects--Topical Terms:

1017719
Biology, Molecular.

Defining the Vibrio harveyi quorum sensing network.
LDR:03123nmm 2200301 4500 001 1812345
005 20060424072429.5
008 130610s2004 eng d
020 $a 0496893300
035 $a (UnM)AAI3142349
035 $a AAI3142349
040 $a UnM $c UnM
100 1 $a Mok, Kenny Chee-Kin. $3 1901894
245 1 0 $a Defining the Vibrio harveyi quorum sensing network.
300 $a 140 p.
500 $a Source: Dissertation Abstracts International, Volume: 65-07, Section: B, page: 3312.
500 $a Adviser: Bonnie Bassler.
502 $a Thesis (Ph.D.)--Princeton University, 2004.
520 $a Vibrio harveyi regulates light production using a cell-cell communication phenomenon called quorum sensing. V. harveyi produces, detects and responds to signaling molecules called autoinducers, whose concentration is correlated with cell density. A multichannel phosphorelay circuit controls light production in response to two autoinducers, AI-1 and AI-2. This thesis shows that AI-1 and AI-2 act synergistically to regulate quorum sensing controlled genes, and that the signaling circuit in V. harveyi acts primarily as a coincidence detector to distinguish between the simultaneous presence of AI-1 and AI-2 and all other conditions (no AI, AI-1 only, or AI-2 alone). Either autoinducer alone can minimally activate or repress target gene expression; however, significant regulation only occurs in the presence of both autoinducers. I propose that a coincidence detector is used to filter out noise from the environment leading to a highly accurate quorum sensing response.
520 $a I identified ten quorum sensing regulated genes, and interestingly, these targets include a metalloprotease (VhpA), components of a Type III secretion system, and a homologue of V. cholerae AphA, which is required to activate the V. cholerae virulence regulon. Thus, V. harveyi controls the expression of virulence factors using quorum sensing. Further study identified a second quorum sensing controlled metalloprotease gene (vhpB) which lies immediately downstream of vhpA. Together, VhpA and VhpB are responsible for all secreted protease activity in V. harveyi.
520 $a A genetic screen in V. harveyi revealed a role for the RNA chaperone Hfq in the repression of quorum sensing. Hfq is required at low cell densities to destabilize luxR mRNA, which encodes the master regulator of quorum sensing in V. harveyi, LuxR. Hfq typically functions in conjunction with small regulatory RNAs (sRNAs). An algorithm was developed to identify the sRNA involved in quorum sensing, and four sRNAs were identified. The simultaneous deletion of all four sRNA genes is required to eliminate the repression of quorum sensing at low cell densities. I propose that Hfq, together with the sRNAs, creates an ultrasensitive, switchlike transition from individual to group behavior.
590 $a School code: 0181.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Microbiology. $3 1017734
690 $a 0307
690 $a 0410
710 2 0 $a Princeton University. $3 645579
773 0 $t Dissertation Abstracts International $g 65-07B.
790 1 0 $a Bassler, Bonnie, $e advisor
790 $a 0181
791 $a Ph.D.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3142349