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Modification of commercial blood cul...

Black, Deborah Streck.

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Modification of commercial blood culture media for more rapid and improved detection of Histoplasma capsulatum by the Bact/ALERT microbial detection system.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Modification of commercial blood culture media for more rapid and improved detection of Histoplasma capsulatum by the Bact/ALERT microbial detection system./
Author:	Black, Deborah Streck.
Description:	60 p.
Notes:	Source: Masters Abstracts International, Volume: 42-06, page: 2107.
Contained By:	Masters Abstracts International42-06.
Subject:	Biology, Microbiology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1420215
ISBN:	0496249649

Modification of commercial blood culture media for more rapid and improved detection of Histoplasma capsulatum by the Bact/ALERT microbial detection system.
Black, Deborah Streck.

Modification of commercial blood culture media for more rapid and improved detection of Histoplasma capsulatum by the Bact/ALERT microbial detection system. - 60 p.

Source: Masters Abstracts International, Volume: 42-06, page: 2107.

Thesis (M.S.)--University of Louisville, 2004.

This thesis is an examination of commercially prepared broth medium for the Bact/ALERT Microbial System. The detection and recovery of microorganisms responsible for bacteremia and several fungemia have improved with the development of the continuous monitoring blood culture system. In a previous seeded fungal blood culture study, a system originally designed to detect bacteremia was shown to include some filamentous fungi. One fungus, Histoplasma capsulatum would not trigger a positive signal in the instrument. It was apparent, by positive terminal cultures, that the organism was fungistatic in all the medium tested but could not produce enough CO2 to be detected by the instrument sensors. The objective of this study was to seed various types of media and determine which produced optimal conditions for the growth and rapid detection of Histoplasma capsulatum.

ISBN: 0496249649Subjects--Topical Terms:

1017734
Biology, Microbiology.

Modification of commercial blood culture media for more rapid and improved detection of Histoplasma capsulatum by the Bact/ALERT microbial detection system.
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020 $a 0496249649
035 $a (UnM)AAI1420215
035 $a AAI1420215
040 $a UnM $c UnM
100 1 $a Black, Deborah Streck. $3 1900907
245 1 0 $a Modification of commercial blood culture media for more rapid and improved detection of Histoplasma capsulatum by the Bact/ALERT microbial detection system.
300 $a 60 p.
500 $a Source: Masters Abstracts International, Volume: 42-06, page: 2107.
500 $a Adviser: James W. Snyder.
502 $a Thesis (M.S.)--University of Louisville, 2004.
520 $a This thesis is an examination of commercially prepared broth medium for the Bact/ALERT Microbial System. The detection and recovery of microorganisms responsible for bacteremia and several fungemia have improved with the development of the continuous monitoring blood culture system. In a previous seeded fungal blood culture study, a system originally designed to detect bacteremia was shown to include some filamentous fungi. One fungus, Histoplasma capsulatum would not trigger a positive signal in the instrument. It was apparent, by positive terminal cultures, that the organism was fungistatic in all the medium tested but could not produce enough CO2 to be detected by the instrument sensors. The objective of this study was to seed various types of media and determine which produced optimal conditions for the growth and rapid detection of Histoplasma capsulatum.
520 $a The first chapter describes various aspects of the fungus, including classification, natural habitat, growth characteristics, metabolism and nutrition, physiology of the organism, and pathology of disease. In chapter two, Materials and Methods, five different media blood culture bottles were used to determine baseline growth of Histoplasma capsulatum. The broths were challenged with a standardized inoculum (103, 102, and 101) of the yeast phase of the organism. Appropriate volume of blood, collected from healthy individuals, was added to each seeded culture, and incubated at 35 C in the instrument to confirm the presence of Histoplasma capsulatum or a true negative. In chapter three, some of the media was supplemented with various carbohydrate sources to enhance the growth of the organism. This did not seem be true for this organism.
520 $a In the final assessment, the addition of oxalic acid decreased the detection time by 12--24 hours less than MB medium supplement with blood and enrichment fluid/lysing agent. Recovery rates and instrument detection appeared to be inoculum (103 > 102 > 10 1) and iron dependent.
590 $a School code: 0110.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Cell. $3 1017686
650 4 $a Engineering, Biomedical. $3 1017684
690 $a 0410
690 $a 0379
690 $a 0541
710 2 0 $a University of Louisville. $3 1017614
773 0 $t Masters Abstracts International $g 42-06.
790 1 0 $a Snyder, James W., $e advisor
790 $a 0110
791 $a M.S.
792 $a 2004
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1420215