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Dorsal-ventral patterning of the zeb...

Nguyen, Vu Huu.

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Dorsal-ventral patterning of the zebrafish embryo.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Dorsal-ventral patterning of the zebrafish embryo./
Author:	Nguyen, Vu Huu.
Description:	154 p.
Notes:	Source: Dissertation Abstracts International, Volume: 61-06, Section: B, page: 2879.
Contained By:	Dissertation Abstracts International61-06B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9976460
ISBN:	0599821752

Dorsal-ventral patterning of the zebrafish embryo.
Nguyen, Vu Huu.

Dorsal-ventral patterning of the zebrafish embryo. - 154 p.

Source: Dissertation Abstracts International, Volume: 61-06, Section: B, page: 2879.

Thesis (Ph.D.)--University of Pennsylvania, 2000.

Three zebrafish mutants, <italic>swirl</italic> (<italic> swr</italic>), <italic>somitabun</italic> (<italic>sbn</italic>), and <italic> snailhouse</italic> (<italic>snh</italic>), which exhibit dorsalized phenotypes, have been identified (Mullins et al., 1996). The genes corresponding to these mutants, <italic>bmp2b</italic>/<italic>swr,</italic> <italic>smad5</italic>/<italic> sbn,</italic> and <italic>bmp7</italic>/<italic>snh,</italic> are members of a BMP pathway (B. Schmid and M. Mullins; Hild et al., 1999; Schmid et al., 2000). We show that these mutants display alterations in the expression of markers of cells located in dorsal and ventral regions of the early gastrula. We show that the domains of expression of ventral markers are reduced in these mutants, with a concomitant expansion of dorsal markers into ventral areas. These changes in gene expression occur prior to or early in gastrulation, at time periods prior to cell commitment in the zebrafish (Ho and Kimmel, 1993). These results show that the <italic>swr</italic>/<italic>bmp2b,</italic> <italic> sbn</italic>/<italic>smad5,</italic> and<italic> snh</italic>/<italic>bmp7 </italic> genes are required for early dorsal-ventral (DV) pattern formation of the zebrafish embryo.

ISBN: 0599821752Subjects--Topical Terms:

1017730
Biology, Genetics.

Dorsal-ventral patterning of the zebrafish embryo.
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100 1 $a Nguyen, Vu Huu. $3 1900066
245 1 0 $a Dorsal-ventral patterning of the zebrafish embryo.
300 $a 154 p.
500 $a Source: Dissertation Abstracts International, Volume: 61-06, Section: B, page: 2879.
500 $a Supervisor: Mary C. Mullins.
502 $a Thesis (Ph.D.)--University of Pennsylvania, 2000.
520 $a Three zebrafish mutants, <italic>swirl</italic> (<italic> swr</italic>), <italic>somitabun</italic> (<italic>sbn</italic>), and <italic> snailhouse</italic> (<italic>snh</italic>), which exhibit dorsalized phenotypes, have been identified (Mullins et al., 1996). The genes corresponding to these mutants, <italic>bmp2b</italic>/<italic>swr,</italic> <italic>smad5</italic>/<italic> sbn,</italic> and <italic>bmp7</italic>/<italic>snh,</italic> are members of a BMP pathway (B. Schmid and M. Mullins; Hild et al., 1999; Schmid et al., 2000). We show that these mutants display alterations in the expression of markers of cells located in dorsal and ventral regions of the early gastrula. We show that the domains of expression of ventral markers are reduced in these mutants, with a concomitant expansion of dorsal markers into ventral areas. These changes in gene expression occur prior to or early in gastrulation, at time periods prior to cell commitment in the zebrafish (Ho and Kimmel, 1993). These results show that the <italic>swr</italic>/<italic>bmp2b,</italic> <italic> sbn</italic>/<italic>smad5,</italic> and<italic> snh</italic>/<italic>bmp7 </italic> genes are required for early dorsal-ventral (DV) pattern formation of the zebrafish embryo.
520 $a We also examined markers of ectoderm cells derived from lateral areas of the fate map. We found that many of these markers, and specifically markers of cranial neural crest progenitors (CNCP), are particularly sensitive to a reduction in BMP activity, both in the context of these mutants and also in <italic>in vivo</italic> overexpression studies. We propose that low BMP activity is present in lateral regions of the gastrula, through the interaction of BMP inhibitors from dorsal axial mesoderm and BMPs expressed in ventral regions, and that a low BMP activity level specifies CNCP. Our analysis in these mutants of cell types situated at dorsal and intermediate positions along the DV axis of the spinal cord, another ectodermally-derived structure, also shows a dependence on BMP activity for the development of these cell types. In conclusion, we demonstrate a genetic requirement in the zebrafish for BMP activity in the proper specification of ectodermal tissues derived from ventral and lateral gastrula cell types, including prospective dorsal and intermediate neural cell types of the spinal cord.
590 $a School code: 0175.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
690 $a 0369
690 $a 0307
710 2 0 $a University of Pennsylvania. $3 1017401
773 0 $t Dissertation Abstracts International $g 61-06B.
790 1 0 $a Mullins, Mary C., $e advisor
790 $a 0175
791 $a Ph.D.
792 $a 2000
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9976460