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The role of two linked hairy/Enhance...

Urban, Michael Keith.

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The role of two linked hairy/Enhancer of split-related zebrafish genes, her1 and her7, in segmentation of the paraxial mesoderm.

Record Type:	Electronic resources : Monograph/item
Title/Author:	The role of two linked hairy/Enhancer of split-related zebrafish genes, her1 and her7, in segmentation of the paraxial mesoderm./
Author:	Urban, Michael Keith.
Description:	165 p.
Notes:	Source: Dissertation Abstracts International, Volume: 64-02, Section: B, page: 0530.
Contained By:	Dissertation Abstracts International64-02B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3082439

The role of two linked hairy/Enhancer of split-related zebrafish genes, her1 and her7, in segmentation of the paraxial mesoderm.
Urban, Michael Keith.

The role of two linked hairy/Enhancer of split-related zebrafish genes, her1 and her7, in segmentation of the paraxial mesoderm. - 165 p.

Source: Dissertation Abstracts International, Volume: 64-02, Section: B, page: 0530.

Thesis (Ph.D.)--University of California, Berkeley, 2002.

The formation of somites, metameric divisions of the paraxial mesoderm, is a dynamic process that gives rise to the major segmented structures of the vertebrate body: the axial skeleton, striated muscles, and the dermis. The discovery of the dynamic expression of several vertebrate <italic>hairy/Enhancer of split</italic>-related genes is of great interest as these genes may well be involved in a molecular oscillator linked to segmentation mechanisms in vertebrates. My overall research goal is to investigate the role of <italic> hairy/Enhancer of split</italic>-related genes during zebrafish somitogenesis. The zebrafish somite mutant, <italic>b567</italic>, is a deficiency mutation that deletes two linked <italic>hairy/Enhancer of split</italic>-related cycling genes, <italic>her1</italic> and <italic>her7</italic>, implicated in segmentation of the paraxial mesoderm. In this dissertation, I show evidence that <italic>her1</italic> and <italic>her7</italic> play partially redundant, but also distinct, roles during somite formation in zebrafish. I report here the results of three primary research efforts: (1) I have characterized the morphological segmentation defects in zebrafish embryos deficient for <italic> her1</italic> and <italic>her7</italic> and demonstrate that the <italic> b567</italic> mutant somitic phenotype is due to the loss of <italic>her1 </italic> and <italic>her7</italic> gene function, (2) I have investigated the functional role of <italic>her1</italic> and <italic>her7</italic> in somite boundary formation, anterior-posterior segment polarity, somite differentiation, and segmental organization of the presomitic mesoderm as evidenced by gene expression patterns, and (3) I have examined Notch/Delta gene expression in embryos deficient for <italic>her1</italic> and <italic>her7</italic> and show evidence that Notch/Delta signaling interacts with <italic>her1</italic> and <italic>her7</italic> gene function in the presomitic mesoderm. In conjunction with an analysis of <italic>b567</italic> mutant embryos, I have undertaken a molecular genetic approach using morpholino antisense oligonucleotides to investigate the consequences on somite patterning resulting from a specific inhibition of <italic>her1</italic> and <italic>her7</italic> gene function. Zebrafish embryos deficient for <italic>her1</italic> and <italic>her7</italic> possess defects in morphological somite boundary formation that is mirrored by a disruption in somite anterior-posterior polarity and segmental patterns of gene expression in the presomitic mesoderm. Taken together, my results suggest that <italic>her1</italic> and <italic>her7</italic> are partially redundant components of a Notch/Delta-linked regulatory pathway involved in segmentation of the paraxial mesoderm in zebrafish.Subjects--Topical Terms:

1017730
Biology, Genetics.

The role of two linked hairy/Enhancer of split-related zebrafish genes, her1 and her7, in segmentation of the paraxial mesoderm.
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040 $a UnM $c UnM
100 1 $a Urban, Michael Keith. $3 1900058
245 1 0 $a The role of two linked hairy/Enhancer of split-related zebrafish genes, her1 and her7, in segmentation of the paraxial mesoderm.
300 $a 165 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-02, Section: B, page: 0530.
500 $a Chair: Sharon L. Amacher.
502 $a Thesis (Ph.D.)--University of California, Berkeley, 2002.
520 $a The formation of somites, metameric divisions of the paraxial mesoderm, is a dynamic process that gives rise to the major segmented structures of the vertebrate body: the axial skeleton, striated muscles, and the dermis. The discovery of the dynamic expression of several vertebrate <italic>hairy/Enhancer of split</italic>-related genes is of great interest as these genes may well be involved in a molecular oscillator linked to segmentation mechanisms in vertebrates. My overall research goal is to investigate the role of <italic> hairy/Enhancer of split</italic>-related genes during zebrafish somitogenesis. The zebrafish somite mutant, <italic>b567</italic>, is a deficiency mutation that deletes two linked <italic>hairy/Enhancer of split</italic>-related cycling genes, <italic>her1</italic> and <italic>her7</italic>, implicated in segmentation of the paraxial mesoderm. In this dissertation, I show evidence that <italic>her1</italic> and <italic>her7</italic> play partially redundant, but also distinct, roles during somite formation in zebrafish. I report here the results of three primary research efforts: (1) I have characterized the morphological segmentation defects in zebrafish embryos deficient for <italic> her1</italic> and <italic>her7</italic> and demonstrate that the <italic> b567</italic> mutant somitic phenotype is due to the loss of <italic>her1 </italic> and <italic>her7</italic> gene function, (2) I have investigated the functional role of <italic>her1</italic> and <italic>her7</italic> in somite boundary formation, anterior-posterior segment polarity, somite differentiation, and segmental organization of the presomitic mesoderm as evidenced by gene expression patterns, and (3) I have examined Notch/Delta gene expression in embryos deficient for <italic>her1</italic> and <italic>her7</italic> and show evidence that Notch/Delta signaling interacts with <italic>her1</italic> and <italic>her7</italic> gene function in the presomitic mesoderm. In conjunction with an analysis of <italic>b567</italic> mutant embryos, I have undertaken a molecular genetic approach using morpholino antisense oligonucleotides to investigate the consequences on somite patterning resulting from a specific inhibition of <italic>her1</italic> and <italic>her7</italic> gene function. Zebrafish embryos deficient for <italic>her1</italic> and <italic>her7</italic> possess defects in morphological somite boundary formation that is mirrored by a disruption in somite anterior-posterior polarity and segmental patterns of gene expression in the presomitic mesoderm. Taken together, my results suggest that <italic>her1</italic> and <italic>her7</italic> are partially redundant components of a Notch/Delta-linked regulatory pathway involved in segmentation of the paraxial mesoderm in zebrafish.
590 $a School code: 0028.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Molecular. $3 1017719
690 $a 0369
690 $a 0379
690 $a 0307
710 2 0 $a University of California, Berkeley. $3 687832
773 0 $t Dissertation Abstracts International $g 64-02B.
790 1 0 $a Amacher, Sharon L., $e advisor
790 $a 0028
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3082439