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Genetic analysis of zebrafish head s...

Miller, Craig Thomas.

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Genetic analysis of zebrafish head skeletal development.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Genetic analysis of zebrafish head skeletal development./
Author:	Miller, Craig Thomas.
Description:	288 p.
Notes:	Source: Dissertation Abstracts International, Volume: 62-02, Section: B, page: 0667.
Contained By:	Dissertation Abstracts International62-02B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3003998
ISBN:	0493133305

Genetic analysis of zebrafish head skeletal development.
Miller, Craig Thomas.

Genetic analysis of zebrafish head skeletal development. - 288 p.

Source: Dissertation Abstracts International, Volume: 62-02, Section: B, page: 0667.

Thesis (Ph.D.)--University of Oregon, 2001.

The vertebrate skeleton consists of variously shaped cartilages and bones separated by joints. How, during embryonic development, are different skeletal elements programmed to form their specific shapes? How are joints positioned within the skeleton? To address these questions, I focus on the segmental pharyngeal head skeleton and use a genetic approach in the zebrafish, <italic> Danio rerio</italic>. This genetic analysis revealed that at a surprisingly early stage in the pharyngeal arch primordia, long before cartilage formation, a prepattern is set up, reflected by the expression of developmental regulatory genes. Patterning along each of the three embryonic axes (dorsal/ventral or D/V, anterior/posterior or A/P, and medial/lateral or M/L) is under largely separable genetic control. Along the D/V axis, a localized Sucker/Endothelin-1 (Suc/Et-1) signal specifies joint and ventral fates by positively regulating the expression of two genes, <italic>bapx1</italic> and <italic>dHAND</italic>. <italic> bapx1</italic>, which encodes a homeobox transcription factor, is expressed in the presumptive first arch joint region. An antisense reduction-of-function method demonstrates that <italic>bapx1</italic> is required for the first arch joint and expression of <italic>gdf5</italic> and <italic>chd</italic> in the developing first arch joint. <italic>dHAND</italic>, which encodes a bHLH transcription factor, is required for ventral cartilage and positions the joint by repressing joint fates, revealing a novel mechanism for the positioning of joints. Along the A/P axis, <italic>bimandibular</italic> (<italic>bmd </italic>) specifies arch 2 identity by positively regulating <italic>hoxa2 </italic> and <italic>hoxb2</italic> expression and negatively regulating <italic> bapx1</italic> expression in the second arch. Along the M/L axis, <italic> cyclops</italic>/<italic>nodal</italic> signaling specifies the anterior neurocranium and is required for midline patterning in the first arch. Collectively these results elaborate a complex genetic network controlling the development of the vertebrate pharyngeal cartilages and the joints which separate them. This dissertation includes both my previously published and unpublished co-authored materials.

ISBN: 0493133305Subjects--Topical Terms:

1017730
Biology, Genetics.

Genetic analysis of zebrafish head skeletal development.
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100 1 $a Miller, Craig Thomas. $3 1900038
245 1 0 $a Genetic analysis of zebrafish head skeletal development.
300 $a 288 p.
500 $a Source: Dissertation Abstracts International, Volume: 62-02, Section: B, page: 0667.
500 $a Adviser: Charles B. Kimmel.
502 $a Thesis (Ph.D.)--University of Oregon, 2001.
520 $a The vertebrate skeleton consists of variously shaped cartilages and bones separated by joints. How, during embryonic development, are different skeletal elements programmed to form their specific shapes? How are joints positioned within the skeleton? To address these questions, I focus on the segmental pharyngeal head skeleton and use a genetic approach in the zebrafish, <italic> Danio rerio</italic>. This genetic analysis revealed that at a surprisingly early stage in the pharyngeal arch primordia, long before cartilage formation, a prepattern is set up, reflected by the expression of developmental regulatory genes. Patterning along each of the three embryonic axes (dorsal/ventral or D/V, anterior/posterior or A/P, and medial/lateral or M/L) is under largely separable genetic control. Along the D/V axis, a localized Sucker/Endothelin-1 (Suc/Et-1) signal specifies joint and ventral fates by positively regulating the expression of two genes, <italic>bapx1</italic> and <italic>dHAND</italic>. <italic> bapx1</italic>, which encodes a homeobox transcription factor, is expressed in the presumptive first arch joint region. An antisense reduction-of-function method demonstrates that <italic>bapx1</italic> is required for the first arch joint and expression of <italic>gdf5</italic> and <italic>chd</italic> in the developing first arch joint. <italic>dHAND</italic>, which encodes a bHLH transcription factor, is required for ventral cartilage and positions the joint by repressing joint fates, revealing a novel mechanism for the positioning of joints. Along the A/P axis, <italic>bimandibular</italic> (<italic>bmd </italic>) specifies arch 2 identity by positively regulating <italic>hoxa2 </italic> and <italic>hoxb2</italic> expression and negatively regulating <italic> bapx1</italic> expression in the second arch. Along the M/L axis, <italic> cyclops</italic>/<italic>nodal</italic> signaling specifies the anterior neurocranium and is required for midline patterning in the first arch. Collectively these results elaborate a complex genetic network controlling the development of the vertebrate pharyngeal cartilages and the joints which separate them. This dissertation includes both my previously published and unpublished co-authored materials.
590 $a School code: 0171.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Anatomy. $3 1021727
690 $a 0369
690 $a 0287
710 2 0 $a University of Oregon. $3 958250
773 0 $t Dissertation Abstracts International $g 62-02B.
790 1 0 $a Kimmel, Charles B., $e advisor
790 $a 0171
791 $a Ph.D.
792 $a 2001
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3003998