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Identification, isolation and charac...

Jiwan, Derick Augustin.

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Identification, isolation and characterization of mildew resistance locus O in Rosaceae.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Identification, isolation and characterization of mildew resistance locus O in Rosaceae./
Author:	Jiwan, Derick Augustin.
Description:	113 p.
Notes:	Source: Dissertation Abstracts International, Volume: 72-09, Section: B, page: .
Contained By:	Dissertation Abstracts International72-09B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3460395
ISBN:	9781124721811

Identification, isolation and characterization of mildew resistance locus O in Rosaceae.
Jiwan, Derick Augustin.

Identification, isolation and characterization of mildew resistance locus O in Rosaceae. - 113 p.

Source: Dissertation Abstracts International, Volume: 72-09, Section: B, page: .

Thesis (Ph.D.)--Washington State University, 2011.

The economically important plant family Rosaceae consists of over 100 genera and 3,000 species that include many important fruit, nut, ornamental, and wood crops. Its members represent diverse biological systems which provide high-value nutritional foods and desirable aesthetic and industrial products. Powdery mildew is a major disease of Rosaceae, requiring control by costly and environmentally harmful chemicals. In this study we identify a Mildew resistance locus O (Mlo) gene in sequenced peach (GU189298), plum (GU189300) and apricot (GU189299) bacterial artificial chromosomes (BACs). Comparative analysis of the BACs with Arabidopsis, Populus and Vitis genome sequences identified high degree of synteny with Populus and Vitis but not with Arabidopsis . Inclusion of Prunoideae Mlo genes expanded the clades in phylogenetic analysis with Arabidopsis and Vitis Mlo genes. Peach Mlo (PpMlo1) was further characterized.

ISBN: 9781124721811Subjects--Topical Terms:

1017719
Biology, Molecular.

Identification, isolation and characterization of mildew resistance locus O in Rosaceae.
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020 $a 9781124721811
035 $a (UMI)AAI3460395
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100 1 $a Jiwan, Derick Augustin. $3 1684980
245 1 0 $a Identification, isolation and characterization of mildew resistance locus O in Rosaceae.
300 $a 113 p.
500 $a Source: Dissertation Abstracts International, Volume: 72-09, Section: B, page: .
500 $a Advisers: Amit Dhingra; Doreen Main.
502 $a Thesis (Ph.D.)--Washington State University, 2011.
520 $a The economically important plant family Rosaceae consists of over 100 genera and 3,000 species that include many important fruit, nut, ornamental, and wood crops. Its members represent diverse biological systems which provide high-value nutritional foods and desirable aesthetic and industrial products. Powdery mildew is a major disease of Rosaceae, requiring control by costly and environmentally harmful chemicals. In this study we identify a Mildew resistance locus O (Mlo) gene in sequenced peach (GU189298), plum (GU189300) and apricot (GU189299) bacterial artificial chromosomes (BACs). Comparative analysis of the BACs with Arabidopsis, Populus and Vitis genome sequences identified high degree of synteny with Populus and Vitis but not with Arabidopsis . Inclusion of Prunoideae Mlo genes expanded the clades in phylogenetic analysis with Arabidopsis and Vitis Mlo genes. Peach Mlo (PpMlo1) was further characterized.
520 $a To test function of PpMlo1, the Fragaria x ananassa LF9 line was transformed with pDAJ3 (Mlo expressed in sense orientation) and pDAJ4 (Mlo expressed in antisense orientation) using Agrobacterium-mediated transformation. Transgenic plants were challenged with Podosphaera macularis conidia, and spore germination and mycelia growth were determined after 72h. Results obtained from the pathogen challenge experiment lead us to infer that antisense repression of the Fragaria Mlo homolog results in conferring resistance to the Fragaria-specific pathogen. Our results indicate that silencing of Mlo is potentially a viable strategy in imparting resistance to powdery mildew within Rosaceae, and that Fragaria can be used as a host to test the function of genes derived from related tree species. To the best of our knowledge, this work is the first attempt to test the potential of using a Mlo -based resistance strategy to combat powdery mildew in Rosaceae.
590 $a School code: 0251.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Agriculture, Plant Pathology. $3 1028950
690 $a 0307
690 $a 0369
690 $a 0480
710 2 $a Washington State University. $b Molecular Plant Sciences. $3 1684981
773 0 $t Dissertation Abstracts International $g 72-09B.
790 1 0 $a Dhingra, Amit, $e advisor
790 1 0 $a Main, Doreen, $e advisor
790 1 0 $a Poovaiah, B.W. $e committee member
790 1 0 $a Thomashow, Linda $e committee member
790 1 0 $a Gill, Kulvinder $e committee member
790 $a 0251
791 $a Ph.D.
792 $a 2011
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3460395