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Dissection and characterization of t...

Sriariyanun, Malinee.

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Dissection and characterization of the ax21 regulatory network .

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Dissection and characterization of the ax21 regulatory network ./
Author:	Sriariyanun, Malinee.
Description:	184 p.
Notes:	Source: Dissertation Abstracts International, Volume: 72-08, Section: B, page: .
Contained By:	Dissertation Abstracts International72-08B.
Subject:	Agriculture, Plant Pathology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3456868
ISBN:	9781124666235

Dissection and characterization of the ax21 regulatory network .
Sriariyanun, Malinee.

Dissection and characterization of the ax21 regulatory network . - 184 p.

Source: Dissertation Abstracts International, Volume: 72-08, Section: B, page: .

Thesis (Ph.D.)--University of California, Davis, 2011.

Xanthomonas oryzae pv. oryzae ( Xoo) is a bacterial pathogen of rice, the worldwide staple and grass model. Xoo invades rice vascular tissues to cause bacterial leaf blight, a serious disease of rice in many parts of the world. An effective method to control the disease is by using resistant rice cultivars. The rice Xa21 gene confers immunity to most strains of Xoo. Liquid chromatography--tandem mass spectrometry analysis (LC-MS/MS) of biologically active fractions from Xoo supernatants leads to the identification of a 194--amino acid protein designated Ax21 (Activator of XA21-mediated immunity). A sulfated, 17--amino acid synthetic peptide (axYS22) derived from the N-terminal region of Ax21 is sufficient for activity, whereas peptides lacking tyrosine sulfation are biologically inactive. axYS22 is 100% conserved in all sequenced Xanthomonas species. Ax21 is a quorum sensing factor that controls density-dependent expression of genes involved in motility, c-di-GMP turnover, cell aggregation, and biofilm formation. Additionally, two response regulators RaxR and PhoP control ax21 expression in opposite manners. The putative cognate sensor kinase for RaxR is called RaxH. The reduction of biofilm production in PXO99DeltaraxH, and the failure of this strain respond to addition of exogenous Ax21 protein suggests that RaxH could be the Ax21 receptor. This work reveals the regulatory mechanisms governing peptide-mediated quorum sensing in the Gram-negative bacterium, Xoo, a process previously thought to be restricted to Gram-positive bacteria.

ISBN: 9781124666235Subjects--Topical Terms:

1028950
Agriculture, Plant Pathology.

Dissection and characterization of the ax21 regulatory network .
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020 $a 9781124666235
035 $a (UMI)AAI3456868
035 $a AAI3456868
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100 1 $a Sriariyanun, Malinee. $3 1684949
245 1 0 $a Dissection and characterization of the ax21 regulatory network .
300 $a 184 p.
500 $a Source: Dissertation Abstracts International, Volume: 72-08, Section: B, page: .
500 $a Adviser: Pamela Ronald.
502 $a Thesis (Ph.D.)--University of California, Davis, 2011.
520 $a Xanthomonas oryzae pv. oryzae ( Xoo) is a bacterial pathogen of rice, the worldwide staple and grass model. Xoo invades rice vascular tissues to cause bacterial leaf blight, a serious disease of rice in many parts of the world. An effective method to control the disease is by using resistant rice cultivars. The rice Xa21 gene confers immunity to most strains of Xoo. Liquid chromatography--tandem mass spectrometry analysis (LC-MS/MS) of biologically active fractions from Xoo supernatants leads to the identification of a 194--amino acid protein designated Ax21 (Activator of XA21-mediated immunity). A sulfated, 17--amino acid synthetic peptide (axYS22) derived from the N-terminal region of Ax21 is sufficient for activity, whereas peptides lacking tyrosine sulfation are biologically inactive. axYS22 is 100% conserved in all sequenced Xanthomonas species. Ax21 is a quorum sensing factor that controls density-dependent expression of genes involved in motility, c-di-GMP turnover, cell aggregation, and biofilm formation. Additionally, two response regulators RaxR and PhoP control ax21 expression in opposite manners. The putative cognate sensor kinase for RaxR is called RaxH. The reduction of biofilm production in PXO99DeltaraxH, and the failure of this strain respond to addition of exogenous Ax21 protein suggests that RaxH could be the Ax21 receptor. This work reveals the regulatory mechanisms governing peptide-mediated quorum sensing in the Gram-negative bacterium, Xoo, a process previously thought to be restricted to Gram-positive bacteria.
590 $a School code: 0029.
650 4 $a Agriculture, Plant Pathology. $3 1028950
690 $a 0480
710 2 $a University of California, Davis. $b Plant Pathology. $3 1676911
773 0 $t Dissertation Abstracts International $g 72-08B.
790 1 0 $a Ronald, Pamela, $e advisor
790 1 0 $a Coaker, Gitta $e committee member
790 1 0 $a Tsolis, Renee $e committee member
790 $a 0029
791 $a Ph.D.
792 $a 2011
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3456868