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Studies of the interphotoreceptor re...

Parker, Ryan.

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Studies of the interphotoreceptor retinoid-binding protein (IRBP) in the cone visual cycle.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Studies of the interphotoreceptor retinoid-binding protein (IRBP) in the cone visual cycle./
作者:	Parker, Ryan.
面頁冊數:	92 p.
附註:	Source: Dissertation Abstracts International, Volume: 72-06, Section: B, page: .
Contained By:	Dissertation Abstracts International72-06B.
標題:	Health Sciences, Ophthalmology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3451919
ISBN:	9781124575094

Studies of the interphotoreceptor retinoid-binding protein (IRBP) in the cone visual cycle.
Parker, Ryan.

Studies of the interphotoreceptor retinoid-binding protein (IRBP) in the cone visual cycle. - 92 p.

Source: Dissertation Abstracts International, Volume: 72-06, Section: B, page: .

Thesis (Ph.D.)--Medical University of South Carolina, 2011.

Rod and cone photoreceptors detect light when 11- cis retinal in the outer segments is isomerized to all-trans retinal to initiate phototransduction. For phototransduction to begin, 11-cis retinal must be consumed, and for it to continue, the all-trans retinal photoproduct must be converted back to 11-cis retinal via a series of enzymatic steps known as the visual cycle. Two separate visual cycles are thought to exist in the mammalian retina. In the classical visual cycle, all-trans retinal is reduced to all-trans retinol in the outer segment, crosses the sub-retinal space to the retinal pigment epithelium (RPE), and is converted back to 11-cis retinal before returning to the photoreceptors. In the second visual cycle, the cone visual cycle, all-trans retinol leaves the photoreceptors, crosses the sub-retinal space, and enters Muller cells, where it is isomerized to 11-cis retinol. 11- cis Retinol is then returned across the sub-retinal space to cones, which have the unique ability to oxidize 11-cis retinol to 11-cis retinal. As such, both cycles depend on the efficient passage of poorly soluble retinoids through the aqueous sub-retinal space. The interphotoreceptor retinoid-binding protein (IRBP) is a large retinoid-binding secreted by the photoreceptors into the sub-retinal space. Because of its location in the sub-retinal space, IRBP is thought to facilitate retinoid transport in the visual cycle, but studies in Irbp -/- mice have shown that the classical visual cycle functions normally. The purpose of our current work is to explore the role of IRBP in the cone visual cycle. Using Irbp-/- mice, we found that cone dysfunction is present in Irbp-/- mice and that the underlying etiology is a cone-specific retinoid deficiency. Additionally, we found that cones in Irbp-/- mice function similar to Wt mice in dark and that cone dysfunction is exacerbated by the presence of background light. Because cones and the cone visual cycle must function normally in the presence of background illumination, these findings suggest that a cone visual cycle deficit is present in Irbp -/- mice. Finally, we used isolated retinae to determine how IRBP facilitates the cone visual cycle. We found that IRBP is able to efficiently deliver 11-cis retinol to cones for oxidation to 11- cis retinal. Together, these findings suggest a novel role for the IRBP in the cone visual cycle. Specifically, IRBP is important for protecting 11-cis retinol as it is transferred from Muller cells to cones. As such, cones utilize IRBP to take advantage of their privileged supply of chromophore, and normal cone function depends on the presence of IRBP.

ISBN: 9781124575094Subjects--Topical Terms:

1019445
Health Sciences, Ophthalmology.

Studies of the interphotoreceptor retinoid-binding protein (IRBP) in the cone visual cycle.
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