東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Regulation of apoptosis in the Caeno...

Nehme, Ralda.

Linked to FindBook

Google Book

Amazon

博客來

Regulation of apoptosis in the Caenorhabditis elegans nervous system.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Regulation of apoptosis in the Caenorhabditis elegans nervous system./
Author:	Nehme, Ralda.
Description:	161 p.
Notes:	Source: Dissertation Abstracts International, Volume: 71-05, Section: B, page: 2807.
Contained By:	Dissertation Abstracts International71-05B.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3398937
ISBN:	9781109733846

Regulation of apoptosis in the Caenorhabditis elegans nervous system.
Nehme, Ralda.

Regulation of apoptosis in the Caenorhabditis elegans nervous system. - 161 p.

Source: Dissertation Abstracts International, Volume: 71-05, Section: B, page: 2807.

Thesis (Ph.D.)--Dartmouth College, 2010.

Most of the cells that die during the development of a C. elegans hermaphrodite do so within 30 min after being generated. In these cells, the pro-caspase proCED-3 is inherited from progenitors and the transcriptional upregulation of the BH3-only gene egl-1 is thought to be sufficient for apoptosis induction. For instance, the NSM sister cells die &sim;20 min after being generated, while their sisters, the NSMs, survive and differentiate into serotonergic neurons. Using forward and reverse genetic approaches, I characterized factors previously not implicated in the NSM sister cell death that regulate egl-1 activity to specify the cell-death fate of the NSM sister cells.

ISBN: 9781109733846Subjects--Topical Terms:

1017730
Biology, Genetics.

Regulation of apoptosis in the Caenorhabditis elegans nervous system.
LDR:03182nam 2200325 4500 001 1404536
005 20111130124029.5
008 130515s2010 ||||||||||||||||| ||eng d
020 $a 9781109733846
035 $a (UMI)AAI3398937
035 $a AAI3398937
040 $a UMI $c UMI
100 1 $a Nehme, Ralda. $3 1683864
245 1 0 $a Regulation of apoptosis in the Caenorhabditis elegans nervous system.
300 $a 161 p.
500 $a Source: Dissertation Abstracts International, Volume: 71-05, Section: B, page: 2807.
500 $a Adviser: Barbara Conradt.
502 $a Thesis (Ph.D.)--Dartmouth College, 2010.
520 $a Most of the cells that die during the development of a C. elegans hermaphrodite do so within 30 min after being generated. In these cells, the pro-caspase proCED-3 is inherited from progenitors and the transcriptional upregulation of the BH3-only gene egl-1 is thought to be sufficient for apoptosis induction. For instance, the NSM sister cells die &sim;20 min after being generated, while their sisters, the NSMs, survive and differentiate into serotonergic neurons. Using forward and reverse genetic approaches, I characterized factors previously not implicated in the NSM sister cell death that regulate egl-1 activity to specify the cell-death fate of the NSM sister cells.
520 $a A few cells that die during C. elegans development, however, survive for more than 30 min before they die. For example, the four CEM neurons die only &sim;150 min after being generated. Furthermore, the CEMs die in hermaphrodites but not males or masculinized hermaphrodites. I found that in the CEMs, the transcriptional activation of both the egl-1 and ced-3 gene is necessary for apoptosis induction. In addition, the Bar homeodomain transcription factor CEH-30 represses egl-1 and ced-3 transcription in the CEMs, thereby causing their survival. I also identified three genes, unc-86, lrs-1 and unc-132, which encode a POU homeodomain transcription factor, a leucyl-tRNA synthetase and a protein with similarity to the mammalian proto-oncoprotein PIM-1, respectively, that promote the expression of the ceh-30 gene in the CEMs. I propose that egl-1 and ced-3 transcription are co-regulated in the CEMs to compensate for limiting proCED-3 levels, caused by proCED-3 turn over. Finally, I present evidence that the life-versus-death decision of the CEMs is also affected by cell migration and cell attachment. Specifically, the inactivation of genes encoding components of the focal adhesion complex results in the inappropriate death of the CEMs in masculinized hermaphrodites, and in the repression of ceh-30 expression. This provides the first evidence that cell non-autonomous, extra-cellular signals control the apoptotic death of neurons in C. elegans.
590 $a School code: 0059.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Cell. $3 1017686
690 $a 0369
690 $a 0379
710 2 $a Dartmouth College. $b Genetics. $3 1058952
773 0 $t Dissertation Abstracts International $g 71-05B.
790 1 0 $a Conradt, Barbara, $e advisor
790 1 0 $a Dunlap, Jay $e committee member
790 1 0 $a Saito, Richard $e committee member
790 1 0 $a Barr, Maureen $e committee member
790 $a 0059
791 $a Ph.D.
792 $a 2010
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3398937