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Transformation and in vitro culture ...

Armenia, Ann Roselle Orillo.

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Transformation and in vitro culture studies to enhance white mold resistance in dry beans (Phaseolus vulgaris L.).

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Transformation and in vitro culture studies to enhance white mold resistance in dry beans (Phaseolus vulgaris L.)./
Author:	Armenia, Ann Roselle Orillo.
Description:	116 p.
Notes:	Source: Masters Abstracts International, Volume: 44-06, page: 2698.
Contained By:	Masters Abstracts International44-06.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1434334
ISBN:	9780542678769

Transformation and in vitro culture studies to enhance white mold resistance in dry beans (Phaseolus vulgaris L.).
Armenia, Ann Roselle Orillo.

Transformation and in vitro culture studies to enhance white mold resistance in dry beans (Phaseolus vulgaris L.). - 116 p.

Source: Masters Abstracts International, Volume: 44-06, page: 2698.

Thesis (M.S.)--Michigan State University, 2006.

White mold, caused by Sclerotinia sclerotiorum, is a serious yield limiting disease in dry beans. Based on evidence that identifies oxalic acid as the major pathogenicity factor for white mold, this study aimed to engineer enhanced white mold resistance in dry beans by overexpressing the wheat oxalate oxidase gene, gf-2.8, using particle bombardment and electrotransformation approaches; and to evaluate effects of cytokinin and particle bombardment on the survival and growth of three bean cultivars in vitro. A total of 1,150 Matterhorn and Olathe bean plants were putatively transformed using the electrotransformation procedure. Screening for bar identified herbicide resistant plants (T1) and PCR analysis confirmed the integration of gf-2.8 in 18 Matterhorn and 11 Olathe plants, the majority of which resulted from Hormone (identity preserved) pretreatments of the apical meristems prior to electrotransformation. Four T2 gf-2.8 PCR positive plants were generated and the expression of gf-2.8 was confirmed in 3 T2 plants through RT-PCR. Indirect tests of T2 and T3 plants showed increased H2O2 production and reduction in lesion size suggesting enhanced white mold resistance. In vitro culture studies of three dry bean cultivars showed that 6-benzylaminopurine in the shoot regeneration media caused a reduction in shoot growth and lengthened the culture time requirement. Bombardment injuries to the cultivar Olathe causes a significant decrease in shoot growth and longer culture time. Red Hawk was observed to be a more suitable cultivar for in vitro culture and transformation efforts of dry beans with particle bombardment.

ISBN: 9780542678769Subjects--Topical Terms:

1017730
Biology, Genetics.

Transformation and in vitro culture studies to enhance white mold resistance in dry beans (Phaseolus vulgaris L.).
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100 1 $a Armenia, Ann Roselle Orillo. $3 1682857
245 1 0 $a Transformation and in vitro culture studies to enhance white mold resistance in dry beans (Phaseolus vulgaris L.).
300 $a 116 p.
500 $a Source: Masters Abstracts International, Volume: 44-06, page: 2698.
500 $a Adviser: James D. Kelly.
502 $a Thesis (M.S.)--Michigan State University, 2006.
520 $a White mold, caused by Sclerotinia sclerotiorum, is a serious yield limiting disease in dry beans. Based on evidence that identifies oxalic acid as the major pathogenicity factor for white mold, this study aimed to engineer enhanced white mold resistance in dry beans by overexpressing the wheat oxalate oxidase gene, gf-2.8, using particle bombardment and electrotransformation approaches; and to evaluate effects of cytokinin and particle bombardment on the survival and growth of three bean cultivars in vitro. A total of 1,150 Matterhorn and Olathe bean plants were putatively transformed using the electrotransformation procedure. Screening for bar identified herbicide resistant plants (T1) and PCR analysis confirmed the integration of gf-2.8 in 18 Matterhorn and 11 Olathe plants, the majority of which resulted from Hormone (identity preserved) pretreatments of the apical meristems prior to electrotransformation. Four T2 gf-2.8 PCR positive plants were generated and the expression of gf-2.8 was confirmed in 3 T2 plants through RT-PCR. Indirect tests of T2 and T3 plants showed increased H2O2 production and reduction in lesion size suggesting enhanced white mold resistance. In vitro culture studies of three dry bean cultivars showed that 6-benzylaminopurine in the shoot regeneration media caused a reduction in shoot growth and lengthened the culture time requirement. Bombardment injuries to the cultivar Olathe causes a significant decrease in shoot growth and longer culture time. Red Hawk was observed to be a more suitable cultivar for in vitro culture and transformation efforts of dry beans with particle bombardment.
590 $a School code: 0128.
650 4 $a Biology, Genetics. $3 1017730
690 $a 0369
710 2 $a Michigan State University. $3 676168
773 0 $t Masters Abstracts International $g 44-06.
790 1 0 $a Kelly, James D., $e advisor
790 $a 0128
791 $a M.S.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1434334