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Gene transfer, gene expression, and ...

Tian, Lining.

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Gene transfer, gene expression, and somatic embryogenesis in two legume species: Soybean and alfalfa.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Gene transfer, gene expression, and somatic embryogenesis in two legume species: Soybean and alfalfa./
作者:	Tian, Lining.
面頁冊數:	245 p.
附註:	Source: Dissertation Abstracts International, Volume: 57-06, Section: B, page: 3582.
Contained By:	Dissertation Abstracts International57-06B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NN08856
ISBN:	9780612088566

Gene transfer, gene expression, and somatic embryogenesis in two legume species: Soybean and alfalfa.
Tian, Lining.

Gene transfer, gene expression, and somatic embryogenesis in two legume species: Soybean and alfalfa. - 245 p.

Source: Dissertation Abstracts International, Volume: 57-06, Section: B, page: 3582.

Thesis (Ph.D.)--Carleton University (Canada), 1995.

Long-day photoperiod-adapted (LDPA) soybean germplasms were screened for their ability to form somatic embryos. The 20 cultivars screened formed between 1 and 7 embryos per cotyledon with between 18% and 98% of the cotyledons responding. At least one ancestral line, 840-7-3, was prominent in the genetic background of the varieties which showed a high degree of embryogenesis. Differential responses were found for two embryogenesis systems. The development of somatic embryogenesis system for LSPA soybean will provide a base for many pure and applied research studies.

ISBN: 9780612088566Subjects--Topical Terms:

1017719
Biology, Molecular.

Gene transfer, gene expression, and somatic embryogenesis in two legume species: Soybean and alfalfa.
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245 1 0 $a Gene transfer, gene expression, and somatic embryogenesis in two legume species: Soybean and alfalfa.
300 $a 245 p.
500 $a Source: Dissertation Abstracts International, Volume: 57-06, Section: B, page: 3582.
500 $a Advisers: J. A. Webb; D. C. W. Brown.
502 $a Thesis (Ph.D.)--Carleton University (Canada), 1995.
520 $a Long-day photoperiod-adapted (LDPA) soybean germplasms were screened for their ability to form somatic embryos. The 20 cultivars screened formed between 1 and 7 embryos per cotyledon with between 18% and 98% of the cotyledons responding. At least one ancestral line, 840-7-3, was prominent in the genetic background of the varieties which showed a high degree of embryogenesis. Differential responses were found for two embryogenesis systems. The development of somatic embryogenesis system for LSPA soybean will provide a base for many pure and applied research studies.
520 $a A simple particle bombardment device was developed and characterized. In this system, DNA-coated tungsten particles were accelerated directly by pressurized helium without using consumable materials. The device delivers genes into soybean and alfalfa cells as efficiently as a commercial device does. The operating cost and set up time for bombardment was reduced. The development and characterization of this new device greatly facilitate the use and distribution of biolistic approach in many laboratories.
520 $a Abiotic and biotic factors were investigated and studied for their influence on alfalfa transformation using biolistic approach. The explants and developmental stage of the explants in culture strongly influenced GUS gene expression. Different types of media resulted in substantially different levels of transgene expression. The results suggest that petioles at day 5 in culture and developed somatic embryos are superior plants for development of a system for stable transformation of alfalfa.
520 $a Embryogenic suspension cultures of Nattawa were bombarded using the plasmid PBI221.23 carrying HYG and GUS genes. The hygromycin-selected cultures showed GUS gene expression. PCR showed the cultures were positive for the GUS gene. Southern Blot analysis of the cultures indicated that the GUS gene and the HYG gene were incorporated into the plant genome. Somatic embryos developed from these cultures. The transformation system developed will provide potential to transform soybean with agronomically important genes.
520 $a ABA was used to induce desiccation tolerance in somatic embryo of soybean to develop a method which may potentially preserve transformed embryogenic lines. A stably transformed soybean embryogenic line showed tolerance to desiccation under RH 43% for at least three days after maturation of embryos. ABA treatment of the embryogenic culture, at all stages of development, did not result in an induction of desiccation tolerance.
590 $a School code: 0040.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Plant Physiology. $3 1017865
690 $a 0307
690 $a 0817
710 2 $a Carleton University (Canada). $3 1018407
773 0 $t Dissertation Abstracts International $g 57-06B.
790 1 0 $a Webb, J. A., $e advisor
790 1 0 $a Brown, D. C. W., $e advisor
790 $a 0040
791 $a Ph.D.
792 $a 1995
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=NN08856