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Freezing tolerance and dehydrin prot...

Goldsmith, Lee Huntington Trebbien.

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Freezing tolerance and dehydrin protein expression in 'Frontenac' and 'Seyval blanc' grapevine bark and xylem cane tissues during acclimation, midwinter, and deacclimation.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Freezing tolerance and dehydrin protein expression in 'Frontenac' and 'Seyval blanc' grapevine bark and xylem cane tissues during acclimation, midwinter, and deacclimation./
Author:	Goldsmith, Lee Huntington Trebbien.
Description:	99 p.
Notes:	Source: Masters Abstracts International, Volume: 48-03, page: 1461.
Contained By:	Masters Abstracts International48-03.
Subject:	Agriculture, Horticulture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1473210
ISBN:	9781109552676

Freezing tolerance and dehydrin protein expression in 'Frontenac' and 'Seyval blanc' grapevine bark and xylem cane tissues during acclimation, midwinter, and deacclimation.
Goldsmith, Lee Huntington Trebbien.

Freezing tolerance and dehydrin protein expression in 'Frontenac' and 'Seyval blanc' grapevine bark and xylem cane tissues during acclimation, midwinter, and deacclimation. - 99 p.

Source: Masters Abstracts International, Volume: 48-03, page: 1461.

Thesis (M.S.)--Iowa State University, 2009.

Although the cold hardiness of primary buds has been examined for grape cultivars and is used as a principle factor of grapevine cold hardiness, freezing tolerance capacity in the rest of the vine has received relatively little attention. This investigation characterized freezing tolerance and protein expression of 'Frontenac' and 'Seyval blanc' grape cane tissues during the overwintering periods of autumnal cold acclimation, midwinter, and vernal deacclimation including dehydrin proteins that may be used to describe cultivar freezing tolerance. Controlled, laboratory freezing tests were conducted with field acclimated or deacclimated bark and xylem tissues from grape canes. SDS-PAGE profiles and immunoblots for dehydrins were used to qualitatively and quantitatively assess protein expression. 'Frontenac' bark tissues demonstrated a capacity to achieve greater freezing tolerance than xylem tissue in late acclimation and midwinter, which may have contributed to greater overall freezing tolerance during midwinter. On the other hand, 'Seyval blanc' showed a greater capacity for reacclimation after a freezing event during the observed deacclimation period. SDS-PAGE profiles showed a 36-kDa protein expressed during the lowest air temperatures and when the lowest LT50 values for 'Frontenac' tissues were observed. Although the 36-kDa protein was not identified as a dehydrin, proteins at 43, 41, 39, 19, 12 and 10 kDa were confirmed as dehydrins. Tissues that were observed to have the greatest capacity for midwinter freezing tolerance also showed greatest expression of a 39-kDa dehydrin. The 12 and 10-kDa dehydrins were not visible on SDS-PAGE profiles, but were detected by the antidehydrin antibody. A 12-kDa dehydrin appeared unique to 'Frontenac' and a 10-kDa dehydrin was unique to bark profiles. The 36-kDa protein and dehydrins at 39, 12, and 10 kDa had greatest association with high freezing tolerance in grapevine.

ISBN: 9781109552676Subjects--Topical Terms:

1017832
Agriculture, Horticulture.

Freezing tolerance and dehydrin protein expression in 'Frontenac' and 'Seyval blanc' grapevine bark and xylem cane tissues during acclimation, midwinter, and deacclimation.
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020 $a 9781109552676
035 $a (UMI)AAI1473210
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040 $a UMI $c UMI
100 1 $a Goldsmith, Lee Huntington Trebbien. $3 1677041
245 1 0 $a Freezing tolerance and dehydrin protein expression in 'Frontenac' and 'Seyval blanc' grapevine bark and xylem cane tissues during acclimation, midwinter, and deacclimation.
300 $a 99 p.
500 $a Source: Masters Abstracts International, Volume: 48-03, page: 1461.
500 $a Advisers: Gail R. Nonnecke; Paul A. Domoto.
502 $a Thesis (M.S.)--Iowa State University, 2009.
520 $a Although the cold hardiness of primary buds has been examined for grape cultivars and is used as a principle factor of grapevine cold hardiness, freezing tolerance capacity in the rest of the vine has received relatively little attention. This investigation characterized freezing tolerance and protein expression of 'Frontenac' and 'Seyval blanc' grape cane tissues during the overwintering periods of autumnal cold acclimation, midwinter, and vernal deacclimation including dehydrin proteins that may be used to describe cultivar freezing tolerance. Controlled, laboratory freezing tests were conducted with field acclimated or deacclimated bark and xylem tissues from grape canes. SDS-PAGE profiles and immunoblots for dehydrins were used to qualitatively and quantitatively assess protein expression. 'Frontenac' bark tissues demonstrated a capacity to achieve greater freezing tolerance than xylem tissue in late acclimation and midwinter, which may have contributed to greater overall freezing tolerance during midwinter. On the other hand, 'Seyval blanc' showed a greater capacity for reacclimation after a freezing event during the observed deacclimation period. SDS-PAGE profiles showed a 36-kDa protein expressed during the lowest air temperatures and when the lowest LT50 values for 'Frontenac' tissues were observed. Although the 36-kDa protein was not identified as a dehydrin, proteins at 43, 41, 39, 19, 12 and 10 kDa were confirmed as dehydrins. Tissues that were observed to have the greatest capacity for midwinter freezing tolerance also showed greatest expression of a 39-kDa dehydrin. The 12 and 10-kDa dehydrins were not visible on SDS-PAGE profiles, but were detected by the antidehydrin antibody. A 12-kDa dehydrin appeared unique to 'Frontenac' and a 10-kDa dehydrin was unique to bark profiles. The 36-kDa protein and dehydrins at 39, 12, and 10 kDa had greatest association with high freezing tolerance in grapevine.
590 $a School code: 0097.
650 4 $a Agriculture, Horticulture. $3 1017832
650 4 $a Biology, Plant Physiology. $3 1017865
690 $a 0471
690 $a 0817
710 2 $a Iowa State University. $b Horticulture. $3 1671764
773 0 $t Masters Abstracts International $g 48-03.
790 1 0 $a Nonnecke, Gail R., $e advisor
790 1 0 $a Domoto, Paul A., $e advisor
790 1 0 $a Arora, Rajeev $e committee member
790 1 0 $a Knapp, Allen D. $e committee member
790 $a 0097
791 $a M.S.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1473210