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The effect of the glucocorticoid dex...

Nebzydoski, Sarah Jean.

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The effect of the glucocorticoid dexamethasone on the clock gene expression in bovine neutrophils and lymphocytes.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	The effect of the glucocorticoid dexamethasone on the clock gene expression in bovine neutrophils and lymphocytes./
Author:	Nebzydoski, Sarah Jean.
Description:	74 p.
Notes:	Source: Masters Abstracts International, Volume: 48-01, page: 0228.
Contained By:	Masters Abstracts International48-01.
Subject:	Biology, Genetics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1469601
ISBN:	9781109393651

The effect of the glucocorticoid dexamethasone on the clock gene expression in bovine neutrophils and lymphocytes.
Nebzydoski, Sarah Jean.

The effect of the glucocorticoid dexamethasone on the clock gene expression in bovine neutrophils and lymphocytes. - 74 p.

Source: Masters Abstracts International, Volume: 48-01, page: 0228.

Thesis (M.S.)--University of Delaware, 2009.

Glucocorticoid induced suppression of neutrophil and lymphocyte function contributes to cattle disease. Although circadian rhythms drive white blood cell function in other species, the role of circadian rhythms on bovine neutrophil and lymphocyte function is unknown. A study was conducted to determine the influence of the synthetic glucocorticoid dexamethasone on 24 h patterns of gene expression in bovine neutrophils and lymphocytes. Six Holstein steers were injected with either saline (control) or dexamethasone (0.10 mg/kg body weight). Neutrophils and lymphocytes were collected from blood sampled by jugular catheters at 0, 4, 8, 12, 16, 20 and 24 h following administration of treatment. Plasma cortisol and melatonin concentrations were also evaluated at those times. One week later the opposite treatments were administered and samples collected. Photoperiod was controlled with lights on at 0700 h and off at 1700 h and treatment injection occurred at 0700 h. Quantitative real time RT-PCR was used to determine relative mRNA levels of L-selectin , GRalpha, and the clock genes Clock, Bmal1, Per1, Cry1, Cry2 , Rev-erbalpha, and CK1epsilon for neutrophils and Clock, Bmal1, Per1, Per2, Cry1, Cry2 , Rev-erbalpha, and CK1epsilon for lymphocytes. mRNA levels were quantified relative to the expression of the housekeeping genes RPS9 and beta-actin. A mixed model was used to analyze the effects of treatment, time, and their interaction on gene expression and plasma cortisol and melatonin. For neutrophils, treatment increased expression of Per1 and decreased expression of Clock, Cry1, Cry2, Rev-erbalpha, L-selectin, and GRalpha (P<0.05), and tended to affect Bmal1 (P=0.07). Time affected Clock, Per1, Cry1, Rev-erbalpha, and CK1epsilon (P<0.05). An interaction between treatment and time was found for Clock, Cry1, and CK1epsilon (P≤0.05). For lymphocytes, treatment increased relative mRNA levels of Per1 and decreased relative mRNA levels for Cry2 ( P<0.05). Time affected Per1 and Rev-erbalpha (P<0.05). An interaction between treatment and time was observed for Per1 (P<0.05) and tended to affect Per2 and Cry2 ( P<0.10). Dexamethasone decreased plasma cortisol concentration (P<0.05) while time affected plasma melatonin concentration (P<0.05). Plasma melatonin concentration increased after lights out at 1700 h and peaked at 2300 h. The results of this experiment provide preliminary evidence that disruption of circadian rhythms in white blood cells may be a factor in periparturient immunosuppression.

ISBN: 9781109393651Subjects--Topical Terms:

1017730
Biology, Genetics.

The effect of the glucocorticoid dexamethasone on the clock gene expression in bovine neutrophils and lymphocytes.
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100 1 $a Nebzydoski, Sarah Jean. $3 1676988
245 1 4 $a The effect of the glucocorticoid dexamethasone on the clock gene expression in bovine neutrophils and lymphocytes.
300 $a 74 p.
500 $a Source: Masters Abstracts International, Volume: 48-01, page: 0228.
500 $a Adviser: Tanya F. Gressley.
502 $a Thesis (M.S.)--University of Delaware, 2009.
520 $a Glucocorticoid induced suppression of neutrophil and lymphocyte function contributes to cattle disease. Although circadian rhythms drive white blood cell function in other species, the role of circadian rhythms on bovine neutrophil and lymphocyte function is unknown. A study was conducted to determine the influence of the synthetic glucocorticoid dexamethasone on 24 h patterns of gene expression in bovine neutrophils and lymphocytes. Six Holstein steers were injected with either saline (control) or dexamethasone (0.10 mg/kg body weight). Neutrophils and lymphocytes were collected from blood sampled by jugular catheters at 0, 4, 8, 12, 16, 20 and 24 h following administration of treatment. Plasma cortisol and melatonin concentrations were also evaluated at those times. One week later the opposite treatments were administered and samples collected. Photoperiod was controlled with lights on at 0700 h and off at 1700 h and treatment injection occurred at 0700 h. Quantitative real time RT-PCR was used to determine relative mRNA levels of L-selectin , GRalpha, and the clock genes Clock, Bmal1, Per1, Cry1, Cry2 , Rev-erbalpha, and CK1epsilon for neutrophils and Clock, Bmal1, Per1, Per2, Cry1, Cry2 , Rev-erbalpha, and CK1epsilon for lymphocytes. mRNA levels were quantified relative to the expression of the housekeeping genes RPS9 and beta-actin. A mixed model was used to analyze the effects of treatment, time, and their interaction on gene expression and plasma cortisol and melatonin. For neutrophils, treatment increased expression of Per1 and decreased expression of Clock, Cry1, Cry2, Rev-erbalpha, L-selectin, and GRalpha (P<0.05), and tended to affect Bmal1 (P=0.07). Time affected Clock, Per1, Cry1, Rev-erbalpha, and CK1epsilon (P<0.05). An interaction between treatment and time was found for Clock, Cry1, and CK1epsilon (P≤0.05). For lymphocytes, treatment increased relative mRNA levels of Per1 and decreased relative mRNA levels for Cry2 ( P<0.05). Time affected Per1 and Rev-erbalpha (P<0.05). An interaction between treatment and time was observed for Per1 (P<0.05) and tended to affect Per2 and Cry2 ( P<0.10). Dexamethasone decreased plasma cortisol concentration (P<0.05) while time affected plasma melatonin concentration (P<0.05). Plasma melatonin concentration increased after lights out at 1700 h and peaked at 2300 h. The results of this experiment provide preliminary evidence that disruption of circadian rhythms in white blood cells may be a factor in periparturient immunosuppression.
590 $a School code: 0060.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Agriculture, Animal Culture and Nutrition. $3 1017857
690 $a 0369
690 $a 0475
710 2 $a University of Delaware. $b Department of Animal and Food Sciences. $3 1029904
773 0 $t Masters Abstracts International $g 48-01.
790 1 0 $a Gressley, Tanya F., $e advisor
790 1 0 $a Kung, Limin $e committee member
790 1 0 $a Emara, Marlene G. $e committee member
790 $a 0060
791 $a M.S.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1469601