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Genetic, genomic, and transgenic app...

McCord, Per Hilding.

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Genetic, genomic, and transgenic approaches to understand internal heat necrosis in potato.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Genetic, genomic, and transgenic approaches to understand internal heat necrosis in potato./
Author:	McCord, Per Hilding.
Description:	192 p.
Notes:	Source: Dissertation Abstracts International, Volume: 70-10, Section: B, page: 5912.
Contained By:	Dissertation Abstracts International70-10B.
Subject:	Agriculture, Horticulture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3377594
ISBN:	9781109443912

Genetic, genomic, and transgenic approaches to understand internal heat necrosis in potato.
McCord, Per Hilding.

Genetic, genomic, and transgenic approaches to understand internal heat necrosis in potato. - 192 p.

Source: Dissertation Abstracts International, Volume: 70-10, Section: B, page: 5912.

Thesis (Ph.D.)--North Carolina State University, 2009.

Internal heat necrosis (IHN) is a physiological disorder of potato tubers. A multifaceted approach was followed to better understand IHN. This approach entailed three major projects, involving genetic, genomic, and transgenic techniques.

ISBN: 9781109443912Subjects--Topical Terms:

1017832
Agriculture, Horticulture.

Genetic, genomic, and transgenic approaches to understand internal heat necrosis in potato.
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020 $a 9781109443912
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100 1 $a McCord, Per Hilding. $3 1676755
245 1 0 $a Genetic, genomic, and transgenic approaches to understand internal heat necrosis in potato.
300 $a 192 p.
500 $a Source: Dissertation Abstracts International, Volume: 70-10, Section: B, page: 5912.
500 $a Advisers: G. Craig Yencho; Bryon R. Sosinski.
502 $a Thesis (Ph.D.)--North Carolina State University, 2009.
520 $a Internal heat necrosis (IHN) is a physiological disorder of potato tubers. A multifaceted approach was followed to better understand IHN. This approach entailed three major projects, involving genetic, genomic, and transgenic techniques.
520 $a The genetics approach involved developing linkage maps of tetraploid potato using AFLP and SSR markers, and identifying QTL for IHN. Our mapping population consisted of 160 individuals from a cross between 'Atlantic', an IHN-susceptible cultivar, and B1829-5, an IHN-resistant breeding line. Phenotypic data indicated that the distribution of IHN is highly skewed toward resistance. Early foliage maturity was positively correlated with reduced IHN. QTL for resistance to IHN were located in multiple years on chromosomes IV, V, and group VII of 'Atlantic', and on group VII of B1829-5. The QTL explained between 6.7 and 35.2 percent of the variation for mean severity, and from 4.9 to 32.5 percent of the variation for percent IHN incidence. Two IHN-linked markers from chromosomes I and V were also associated with IHN in a second population. The correlation between maturity and IHN may be partially explained by loosely linked QTL on chromosomes II and V. QTL were also detected in this population for tuber dry matter content, specific gravity, skin texture, yield, and flower color.
520 $a The genomic approach entailed a microarray-based gene expression analysis of 'Atlantic' potatoes grown under normal and high temperatures in growth chambers. Although overall levels of IHN were not significantly different between temperatures, IHN symptoms increased between the first and last harvest dates under high temperature. Mixed model analysis identified four genes whose expression was different between high and normal temperatures at the first and last harvest dates. Expression of one gene, F3C3.6, was validated via quantitative RT-PCR (qRT-PCR). F3C.6 showed no difference in expression between harvest dates at high temperature, but was down-regulated over time under normal temperature. Quantitative RT-PCR of F3C3.6 using field-grown potatoes showed no differences between clean and IHN-affected tubers, supporting the hypothesis that down-regulation of this gene over time is associated with resistance to IHN.
520 $a Tuber calcium levels may be involved in the expression of IHN, and the transgenic project was designed to test this association. 'Atlantic' was transformed via Agrobacterium tumefaciens with a maize calreticulin-derived calcium binding peptide (CBP) shown to increase bioavailable calcium in Arabidopsis. Plants were grown under mild heat stress conditions in growth chambers. Transgenic lines generally had higher yields than wild-type plants. Levels of IHN in transgenic tubers were equal to or higher than wild-type tubers. Quantitative RT-PCR of CBP showed higher expression in one transgenic line, but also suggested that differing protein abundance underlies phenotypic differences between transgenic lines. Two native calreticulin genes were assayed by qRT-PCR for evidence of silencing, but none was found. Nutrient analysis of calcium and 11 other nutrients was also performed. Calcium levels in leaves of two transgenic lines were higher than wild-type 'Atlantic'. Leaf levels of magnesium, manganese, sulfur, and sodium were also higher than 'Atlantic' in at least one line. Most of these minerals are involved in photosynthesis; increased amounts in leaves could be responsible for the yield increase. In tubers, mineral levels were not significantly different. It is possible that expression of CBP in tubers is much lower than in leaves, which could explain the failure to detect any mineral differences. The increase in IHN could be due to the interruption of a signal from the leaves, or a subtle effect on tuber minerals that would require larger sample sizes to detect.
590 $a School code: 0155.
650 4 $a Agriculture, Horticulture. $3 1017832
690 $a 0471
710 2 $a North Carolina State University. $3 1018772
773 0 $t Dissertation Abstracts International $g 70-10B.
790 1 0 $a Yencho, G. Craig, $e advisor
790 1 0 $a Sosinski, Bryon R., $e advisor
790 $a 0155
791 $a Ph.D.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3377594