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Understanding the cytokinin N-glucos...

Dwivedi, Somya.

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Understanding the cytokinin N-glucosylation pathway using Arabidopsis Mutants.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Understanding the cytokinin N-glucosylation pathway using Arabidopsis Mutants./
作者:	Dwivedi, Somya.
面頁冊數:	130 p.
附註:	Source: Dissertation Abstracts International, Volume: 70-03, Section: B, page: 1399.
Contained By:	Dissertation Abstracts International70-03B.
標題:	Agriculture, Horticulture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3351330
ISBN:	9781109074734

Understanding the cytokinin N-glucosylation pathway using Arabidopsis Mutants.
Dwivedi, Somya.

Understanding the cytokinin N-glucosylation pathway using Arabidopsis Mutants. - 130 p.

Source: Dissertation Abstracts International, Volume: 70-03, Section: B, page: 1399.

Thesis (Ph.D.)--University of Connecticut, 2009.

N-glucosylation is considered to be a cytokinin inactivation mechanism in plants. We have isolated a mutant in Arabidopsis thaliana, roscovitine-resistant (ror-1) using T-DNA tagged lines that may encode a gene that plays a role in cytokinin N-glucosylation. The amount of N-glucosides was 20% more in ror-1 mutant compared to WT. The cytokinin oxidase activity showed an 11% increase in ror-1 compared to WT in basal media. Additionally, ror-1 plants showed resistance to exogenously supplied aromatic cytokinins (benzyladenine). Our data on relative expression of ROR-1 gene exhibited significant upregulation of ROR-1 gene in ror-1 plants incubated with 5 muM BA for 24 h. The in silico analysis showed that ROR-1 gene encoded a protein consisting of GRAM (Glycosyltransferases Rab-like GTPase activators and Myotubularins) and C2 domains. Here, we report that the ROR-1 gene plays a role in metabolizing active cytokinin pool in plants by specifically affecting aromatic cytokinin metabolism.

ISBN: 9781109074734Subjects--Topical Terms:

1017832
Agriculture, Horticulture.

Understanding the cytokinin N-glucosylation pathway using Arabidopsis Mutants.
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520 $a N-glucosylation is considered to be a cytokinin inactivation mechanism in plants. We have isolated a mutant in Arabidopsis thaliana, roscovitine-resistant (ror-1) using T-DNA tagged lines that may encode a gene that plays a role in cytokinin N-glucosylation. The amount of N-glucosides was 20% more in ror-1 mutant compared to WT. The cytokinin oxidase activity showed an 11% increase in ror-1 compared to WT in basal media. Additionally, ror-1 plants showed resistance to exogenously supplied aromatic cytokinins (benzyladenine). Our data on relative expression of ROR-1 gene exhibited significant upregulation of ROR-1 gene in ror-1 plants incubated with 5 muM BA for 24 h. The in silico analysis showed that ROR-1 gene encoded a protein consisting of GRAM (Glycosyltransferases Rab-like GTPase activators and Myotubularins) and C2 domains. Here, we report that the ROR-1 gene plays a role in metabolizing active cytokinin pool in plants by specifically affecting aromatic cytokinin metabolism.
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