東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Genetically Modified Aedes aegypti f...

Mathur, Geetika.

FindBook

Google Book

Amazon

博客來

Genetically Modified Aedes aegypti for the Control of Dengue Transmission.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Genetically Modified Aedes aegypti for the Control of Dengue Transmission./
作者:	Mathur, Geetika.
面頁冊數:	126 p.
附註:	Source: Dissertation Abstracts International, Volume: 72-01, Section: B, page: .
Contained By:	Dissertation Abstracts International72-01B.
標題:	Biology, Molecular. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3428284
ISBN:	9781124347820

Genetically Modified Aedes aegypti for the Control of Dengue Transmission.
Mathur, Geetika.

Genetically Modified Aedes aegypti for the Control of Dengue Transmission. - 126 p.

Source: Dissertation Abstracts International, Volume: 72-01, Section: B, page: .

Thesis (Ph.D.)--University of California, Irvine, 2010.

Genetics-based vector control strategies based on population replacement are being developed for mosquito-borne diseases including dengue. Population replacement requires controlled sex- and tissue-specific expression of effector genes and development of a drive system to introgress the effector genes in wild mosquito populations. Female mosquito salivary glands are critical sites for expression of effector genes as they play a crucial role in virus transmission. Transcriptome analyses of Aedes aegypti discovered that the 30K genes are expressed exclusively and in great abundance in the salivary glands of adult females. Blast searches of the genome revealed that there are three 30K genes in Ae. aegypti, all present on the same supercontig. Two of these genes, 30K a and 30K b, are separated by a 263 bp intergenic region and are transcribed divergently. Orthology of the 30K genes in the mosquito species was established and the 5' upstream region was scanned for conserved regulatory motifs but no conserved motifs were found. Based on the genomic sequence and Expressed Sequence Tags (EST) data available for the 30K a and b genes, the 5' and 3' untranslated regions (UTRs) of the genes were identified. The functional characterization of the putative bidirectional promoter and control elements was performed in transgenic Ae. Aegypti. The cis-regulatory sequences of the 30K a and 30K b genes were used to express simultaneously an enhanced green fluorescent protein (EGFP) reporter and an anti-dengue effector gene, Mnp, in the salivary glands of female mosquitoes. The expression of the effector gene in female salivary glands resulted in reduced prevalence and mean intensities of virus infection in salivary glands and saliva. Efforts to develop a transposon based gene drive system were made by testing the remobilization potential of three different class II transposable elements Mos1 mariner, Osmar5 and mPing in Ae. aegypti. No remobilization of Mos1 was detected in transgenic females expressing Mos1 transposase in the germ cells. Interplasmid mobility assays for Osmar5 and mPing performed in Ae. aegypti embryos also indicated no movement.

ISBN: 9781124347820Subjects--Topical Terms:

1017719
Biology, Molecular.

Genetically Modified Aedes aegypti for the Control of Dengue Transmission.
LDR:03240nam 2200325 4500 001 1396833
005 20110712090426.5
008 130515s2010 ||||||||||||||||| ||eng d
020 $a 9781124347820
035 $a (UMI)AAI3428284
035 $a AAI3428284
040 $a UMI $c UMI
100 1 $a Mathur, Geetika. $3 1675633
245 1 0 $a Genetically Modified Aedes aegypti for the Control of Dengue Transmission.
300 $a 126 p.
500 $a Source: Dissertation Abstracts International, Volume: 72-01, Section: B, page: .
500 $a Adviser: Anthony A. James.
502 $a Thesis (Ph.D.)--University of California, Irvine, 2010.
520 $a Genetics-based vector control strategies based on population replacement are being developed for mosquito-borne diseases including dengue. Population replacement requires controlled sex- and tissue-specific expression of effector genes and development of a drive system to introgress the effector genes in wild mosquito populations. Female mosquito salivary glands are critical sites for expression of effector genes as they play a crucial role in virus transmission. Transcriptome analyses of Aedes aegypti discovered that the 30K genes are expressed exclusively and in great abundance in the salivary glands of adult females. Blast searches of the genome revealed that there are three 30K genes in Ae. aegypti, all present on the same supercontig. Two of these genes, 30K a and 30K b, are separated by a 263 bp intergenic region and are transcribed divergently. Orthology of the 30K genes in the mosquito species was established and the 5' upstream region was scanned for conserved regulatory motifs but no conserved motifs were found. Based on the genomic sequence and Expressed Sequence Tags (EST) data available for the 30K a and b genes, the 5' and 3' untranslated regions (UTRs) of the genes were identified. The functional characterization of the putative bidirectional promoter and control elements was performed in transgenic Ae. Aegypti. The cis-regulatory sequences of the 30K a and 30K b genes were used to express simultaneously an enhanced green fluorescent protein (EGFP) reporter and an anti-dengue effector gene, Mnp, in the salivary glands of female mosquitoes. The expression of the effector gene in female salivary glands resulted in reduced prevalence and mean intensities of virus infection in salivary glands and saliva. Efforts to develop a transposon based gene drive system were made by testing the remobilization potential of three different class II transposable elements Mos1 mariner, Osmar5 and mPing in Ae. aegypti. No remobilization of Mos1 was detected in transgenic females expressing Mos1 transposase in the germ cells. Interplasmid mobility assays for Osmar5 and mPing performed in Ae. aegypti embryos also indicated no movement.
590 $a School code: 0030.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Entomology. $3 1018619
650 4 $a Biology, Genetics. $3 1017730
690 $a 0307
690 $a 0353
690 $a 0369
710 2 $a University of California, Irvine. $b Biological Sciences - Ph.D. $3 1675588
773 0 $t Dissertation Abstracts International $g 72-01B.
790 1 0 $a James, Anthony A., $e advisor
790 1 0 $a Morrissette, Naomi $e committee member
790 1 0 $a Walsh, Craig M. $e committee member
790 1 0 $a Marsh, J. Lawrence $e committee member
790 $a 0030
791 $a Ph.D.
792 $a 2010
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3428284