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A proteomic approach to identify bio...

Ding, Juan.

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A proteomic approach to identify biomarkers of growth hormone and aging.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	A proteomic approach to identify biomarkers of growth hormone and aging./
Author:	Ding, Juan.
Description:	312 p.
Notes:	Source: Dissertation Abstracts International, Volume: 70-09, Section: B, page: 5315.
Contained By:	Dissertation Abstracts International70-09B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3372300
ISBN:	9781109352429

A proteomic approach to identify biomarkers of growth hormone and aging.
Ding, Juan.

A proteomic approach to identify biomarkers of growth hormone and aging. - 312 p.

Source: Dissertation Abstracts International, Volume: 70-09, Section: B, page: 5315.

Thesis (Ph.D.)--Ohio University, 2009.

The growth hormone (GH)/insulin-like growth factor-1 (IGF-1) axis is an important regulator of longitudinal growth, metabolism and aging. The physiological role of GH is manifested in mouse models with altered GH signaling. For example, bovine (b) GH transgenic mice are giant, lean, insulin resistant and short-lived. In contrast, GH receptor (R) gene knockout (GHR-/-) mice are dwarf, obese, insulin sensitive and long-lived. In order to uncover the biomarkers of GH and aging, two-dimensional gel electrophoresis (2-DE) has been used to analyze the mouse plasma proteome. The bGH, GHR-/- mice and their respective controls at different ages were analyzed. Female GHR-/- and control mice were also analyzed for gender differences.

ISBN: 9781109352429Subjects--Topical Terms:

1017719
Biology, Molecular.

A proteomic approach to identify biomarkers of growth hormone and aging.
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020 $a 9781109352429
035 $a (UMI)AAI3372300
035 $a AAI3372300
040 $a UMI $c UMI
100 1 $a Ding, Juan. $3 1669846
245 1 2 $a A proteomic approach to identify biomarkers of growth hormone and aging.
300 $a 312 p.
500 $a Source: Dissertation Abstracts International, Volume: 70-09, Section: B, page: 5315.
500 $a Adviser: John J. Kopchick.
502 $a Thesis (Ph.D.)--Ohio University, 2009.
520 $a The growth hormone (GH)/insulin-like growth factor-1 (IGF-1) axis is an important regulator of longitudinal growth, metabolism and aging. The physiological role of GH is manifested in mouse models with altered GH signaling. For example, bovine (b) GH transgenic mice are giant, lean, insulin resistant and short-lived. In contrast, GH receptor (R) gene knockout (GHR-/-) mice are dwarf, obese, insulin sensitive and long-lived. In order to uncover the biomarkers of GH and aging, two-dimensional gel electrophoresis (2-DE) has been used to analyze the mouse plasma proteome. The bGH, GHR-/- mice and their respective controls at different ages were analyzed. Female GHR-/- and control mice were also analyzed for gender differences.
520 $a Several biomarkers of aging and GH actions have been discovered. Biomarkers for aging include proteins whose plasma levels increased (isoform(s) of transthyretin (TTR), haptoglobin (Hp), immunoglobulin kappa chain) and decreased (isoform(s) of albumin, serum amyloid A-1 (SAA-1) and peroxiredoxin-2). Biomarkers for chronic GH action include upregulation of apolipoprotein (apo) E, Hp, mannose-binding protein-C, specific isoform(s) of alpha-2 macroglobin (a2m), retinol-binding protein-4 (RBP-4), and downregulation of apoA4, TTR, specific isoforms of apoA1, a2m and RBP-4. Importantly, isoforms of Hp and clusterin increased markedly in bGH mice during aging compared to wild type (WT) mice, possibly indicating an accelerated aging phenotype of these mice. Gender-specific biomarkers include plasma proteins that increased such as isoform(s) of apoE and RBP-4 or decreased such as isoform(s) of clusterin, albumin, Hp and hemoblogin-beta in females. Interestingly, some proteins showed no change during male aging but significant increase (isoform(s) of apoA1, apoA4, TTR and albumin) or decrease (isoform(s) of RBP-4 and albumin) during female aging. In addition, several proteins (Hp, apoE and RBP-4) showed an interaction between genotype (GHR-/- and WT) and gender, suggesting interplay between GH and sex.
520 $a Finally, short-term effects of GH/IGF-1 were studied in mice injected with GH and/or IGF-1. GH injection resulted in upregulation of isoform(s) of apoE, Hp, RBP-4, SAA-1, albumin and a2m; and downregulated isoform(s) of apoA4 and clusterin. For IGF-1, isoform(s) of Hp, albumin and TTR were upregulated. Further, biomarkers differentiating between GH and IGF-1 actions have been revealed, including isoform(s) of apoE and TTR. The biomarkers of GH/IGF-1 injection will provide potential candidates for detecting GH and IGF-1 doping.
590 $a School code: 0167.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Animal Physiology. $3 1017835
650 4 $a Health Sciences, Aging. $3 1669845
650 4 $a Biology, Bioinformatics. $3 1018415
690 $a 0307
690 $a 0433
690 $a 0493
690 $a 0715
710 2 $a Ohio University. $b Department of Biological Sciences. $3 1669847
773 0 $t Dissertation Abstracts International $g 70-09B.
790 1 0 $a Kopchick, John J., $e advisor
790 1 0 $a Berryman, Darlene E. $e committee member
790 1 0 $a Chen, Xiaozhuo $e committee member
790 1 0 $a James, Calvin $e committee member
790 $a 0167
791 $a Ph.D.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3372300