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A primary cilia disease protein netw...

Miller, Julie J.

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A primary cilia disease protein network centered at the centrosome.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	A primary cilia disease protein network centered at the centrosome./
Author:	Miller, Julie J.
Description:	155 p.
Notes:	Source: Dissertation Abstracts International, Volume: 70-07, Section: B, page: 3876.
Contained By:	Dissertation Abstracts International70-07B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3364150
ISBN:	9781109241297

A primary cilia disease protein network centered at the centrosome.
Miller, Julie J.

A primary cilia disease protein network centered at the centrosome. - 155 p.

Source: Dissertation Abstracts International, Volume: 70-07, Section: B, page: 3876.

Thesis (Ph.D.)--Stanford University, 2009.

The patterning of tissues and organisms involves the intricate coordination of tell division, morphogenesis and tell differentiation to produce a spatially and temporally organized population of tells. The proper timing and precise execution of the tell division cycle, which drives the reproduction of tells, is fundamental to this process and is tightly regulated both by extracellular cues and intracellular checkpoint mechanisms. Using protein complex purification and mass spectrometry, we have investigated the control and organization of tell growth in two different settings: (1) regulation of ubiquitin-mediated protein destruction by the Anaphase-Promoting Complex by the Emi1 protein and (2) the formation and function of the primary cilium by ciliadisease proteins.

ISBN: 9781109241297Subjects--Topical Terms:

1017719
Biology, Molecular.

A primary cilia disease protein network centered at the centrosome.
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020 $a 9781109241297
035 $a (UMI)AAI3364150
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100 1 $a Miller, Julie J. $3 1669311
245 1 2 $a A primary cilia disease protein network centered at the centrosome.
300 $a 155 p.
500 $a Source: Dissertation Abstracts International, Volume: 70-07, Section: B, page: 3876.
500 $a Adviser: Peter K. Jackson.
502 $a Thesis (Ph.D.)--Stanford University, 2009.
520 $a The patterning of tissues and organisms involves the intricate coordination of tell division, morphogenesis and tell differentiation to produce a spatially and temporally organized population of tells. The proper timing and precise execution of the tell division cycle, which drives the reproduction of tells, is fundamental to this process and is tightly regulated both by extracellular cues and intracellular checkpoint mechanisms. Using protein complex purification and mass spectrometry, we have investigated the control and organization of tell growth in two different settings: (1) regulation of ubiquitin-mediated protein destruction by the Anaphase-Promoting Complex by the Emi1 protein and (2) the formation and function of the primary cilium by ciliadisease proteins.
520 $a The periodic destruction of mitotic cyclins is triggered by the activation of the Anaphase Promoting Complex/Cyclosome (APC/C) in mitosis. Although the ability of the APC/C to recognize destruction (D-box) substrates oscillates throughout the tell cycle, the mechanism regulating APC/C binding to D-box substrates remains unclear. Our analysis of the Emi1 protein, an inhibitor of the APC/C, elucidated the manner by which the activity of APC/C is maintained low until mitosis. We have found that Emi1 tightly binds both the APC/C and its Cdh1 activator at the D-box receptor site on the APC/C Cdh1, and competes with APC/C substrates for D-box binding. Emi1 itself contains a conserved C-terminal D-box, which provides APC/C binding affinity, and a conserved zinc-binding region (ZBR), which antagonizes APC/C E3 ligase activity independent of tight APC binding. Mutation of the ZBR converts Emi1 into a D-box dependent APC/C substrate. The identification of a direct Emi1-APC/C complex further supports the idea that Emi1 functions as a stabilizing factor for cyclin accumulation. The combination of a degron/E3 recognition site and an anti-ligase function in Emi1 suggests a general model for how E3 substrates evolve to become pseudosubstrate inhibitors.
520 $a Our analysis of a number of cilia disease proteins, or cystproteins, suggests that the primary cilium and centrosome have an important role in the timing and spatial organization of tell division. The ciliopathies are a group of rare, genetically heterogeneous diseases thought to be etiologically linked to dysfunction of the primary cilium, a microtubule-based organelle found on almost all vertebrate tells. These diseases exhibit common clinical phenotypes that include renal cystic disease, neurological disorders, polydactyly, obesity and retinal degeneration. Over the last decade, tremendous progress has been made identifying the causative mutations, yet we are only beginning to understand the events leading to the development of disease. In order to better understand both the mechanisms involved in disease pathogenesis and the basic biology of the primary cilium and its role in organizing tell division, we have used proteomics to study disease proteins linked to the highly related ciliopathies Nephronophthisis, Joubert Syndrome and Meckel-Gruber Syndrome. This approach has revealed an extensive set of connections among disease proteins, enabling us to build a cystoprotein network model that greatly simplifies our understanding of the physical and function relationships among this set of proteins. We went on to further investigate one node of the cystoprotein network, composed of NPHP5, NPHP6/CEP290 and NPHP2/Inversin. These three proteins, and the novel interacting proteins Usp9x and Nek8, co-localize at the centrosome and function cooperatively in this compartment. Depletion of NPHP5, NPHP6 and NPHP2 results in striking defects in cilia formation and, additionally, perturbs other centrosome-related functions, including cytokinesis. We propose a model in which NPHP5, NPHP6 and NPHP2 mutually depend on each other for localization and action at the centrosome. We are currently investigating the role of a number of novel interacting proteins for insight into the ciliogenic function of the NPHP5-NPHP6-NPHP2 node.
590 $a School code: 0212.
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Cell. $3 1017686
690 $a 0307
690 $a 0369
690 $a 0379
710 2 $a Stanford University. $3 754827
773 0 $t Dissertation Abstracts International $g 70-07B.
790 1 0 $a Jackson, Peter K., $e advisor
790 $a 0212
791 $a Ph.D.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3364150