東華大學圖書館 |

PCR applications = protocols for functional genomics /

Record Type:	Electronic resources : Monograph/item
Title/Author:	PCR applications/ edited by Michael A. Innis, David H. Gelfand, John J. Sninsky.{me_controlnum}
Reminder of title:	protocols for functional genomics /
other author:	Sninsky, John J.
Published:	San Diego :Academic Press, : c1999.,
Description:	1 online resource (xviii, 566 p., [3] p. of plates) :ill. (some col.)
[NT 15003449]:	pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity -- Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- pt. 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR -- Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- pt. 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research -- Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- pt. 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome -- Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning.
Subject:	Polymerase chain reaction. -
Online resource:	http://www.sciencedirect.com/science/book/9780123721853
ISBN:	9780123721853

PCR applications = protocols for functional genomics /
PCR applicationsprotocols for functional genomics /[electronic resource] :edited by Michael A. Innis, David H. Gelfand, John J. Sninsky.{me_controlnum} - San Diego :Academic Press,c1999. - 1 online resource (xviii, 566 p., [3] p. of plates) :ill. (some col.)

Includes bibliographical references and index.

pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity -- Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- pt. 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR -- Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- pt. 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research -- Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- pt. 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome -- Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning.

PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. Entries provide information on: * Nomenclature * Expression * Sequence analysis * Structure and function * Electrophysiology * Parmacology * Information retrieval.

ISBN: 9780123721853

Source: 78885:78885Elsevier Science & Technologyhttp://www.sciencedirect.comSubjects--Topical Terms:

518049
Polymerase chain reaction.
Index Terms--Genre/Form:

542853
Electronic books.

LC Class. No.: QP606.D46 / P346 1999eb

Dewey Class. No.: 572.8/6

National Library of Medicine Call No.: 1999 E-525

PCR applications = protocols for functional genomics /
LDR:04572cmm 2200337Ia 4500 001 1326067
005 20120813080621.0
006 m d
007 cr cn|||||||||
008 130122s1999 enkaf ob 001 0 eng d
020 $a 9780123721853
020 $a 0123721857
029 1 $a NZ1 $b 12433370
029 1 $a AU@ $b 000048129801
035 $a ocn162573823
037 $a 78885:78885 $b Elsevier Science & Technology $n http://www.sciencedirect.com
040 $a OPELS $b eng $c OPELS $d OKU $d OCLCQ $d IDEBK $d OCLCQ
049 $a NTYA
050 4 $a QP606.D46 $b P346 1999eb
060 4 $a 1999 E-525
060 4 $a QH 450.3 $b P347 1999
082 0 4 $a 572.8/6 $2 22
245 0 0 $a PCR applications $h [electronic resource] : $b protocols for functional genomics / $c edited by Michael A. Innis, David H. Gelfand, John J. Sninsky.{me_controlnum}
260 $a San Diego : $b Academic Press, $c c1999.
300 $a 1 online resource (xviii, 566 p., [3] p. of plates) : $b ill. (some col.)
504 $a Includes bibliographical references and index.
505 0 $a pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity -- Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- pt. 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR -- Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- pt. 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research -- Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- pt. 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome -- Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning.
520 $a PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily. PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. Entries provide information on: * Nomenclature * Expression * Sequence analysis * Structure and function * Electrophysiology * Parmacology * Information retrieval.
588 $a Description based on print version record.
650 0 $a Polymerase chain reaction. $3 518049
650 0 $a Gene amplification. $3 533118
650 0 $a Polymerase chain reaction $v Laboratory manuals. $3 1615181
650 0 $a Gene amplification $v Laboratory manuals. $3 1615182
650 0 $a Genomics $3 1512923
650 1 2 $a Polymerase Chain Reaction $x methods. $3 595460
650 2 2 $a Genetic Engineering. $3 600566
650 4 $a Genomes. $3 592593
650 4 $a Polymerase chain reaction $x Diagnostic use. $3 833894
650 6 $a R�eaction en cha�ine de la polym�erase. $3 1615183
650 6 $a Amplification g�enique. $3 1615184
650 6 $a R�eaction en cha�ine de la polym�erase $x Manuels de laboratoire. $3 1615185
650 6 $a Amplification g�enique $v Manuels de laboratoire. $3 1615186
650 1 7 $a Polymerase kettingreactie. $2 gtt $3 1615187
650 1 7 $a Genoom. $2 gtt $3 1615188
655 4 $a Electronic books. $2 lcsh $3 542853
700 1 $a Sninsky, John J. $3 533115
700 1 $a Innis, Michael A. $3 533116
700 1 $a Gelfand, David H. $3 533117
776 0 8 $i Print version: $t PCR applications. $d San Diego : Academic Press, c1999 $z 0123721857 $z 9780123721853 $w (DLC) 99211203 $w (OCoLC)41339460
856 4 0 $3 ScienceDirect $u http://www.sciencedirect.com/science/book/9780123721853
938 $a Ingram Digital eBook Collection $b IDEB $n 176378