東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

RNA methodologies = a laboratory gui...

Farrell, Robert E.{me_controlnum}

FindBook

Google Book

Amazon

博客來

RNA methodologies = a laboratory guide for isolation and characterization /

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	RNA methodologies/ Robert E. Farrell, Jr.
其他題名:	a laboratory guide for isolation and characterization /
作者:	Farrell, Robert E.{me_controlnum}
出版者:	Amsterdam ;Elsevier/Academic Press, : c2005.,
面頁冊數:	1 online resource (xxv, 767 p.) :ill.
內容註:	RNA and the cellular biochemistry revisited -- Transcription and the organization of eukaryotic genes -- Messenger RNA -- Resilient ribonucleases -- RNA isolation strategies -- The truth about tissues -- Isolation of polyadenylated RNA -- Quality control for RNA preparations -- Dot blot analysis -- Electrophoresis of RNA -- Photodocumentation and image analysis -- Northern analysis -- Nucleic acid probe technology -- Practical nucleic acid hybridization -- Principles of detection -- Quantification of specific mRNAs by nuclease protection -- Analysis of nuclear RNA -- cDNA synthesis -- RT-PCR -- Quantitative PCR techniques -- Transcript subtraction methods -- mRNA differential display -- High-throughput analysis of gene expression -- RNA interference : targeted gene silencing -- Genomes, transcriptomes, proteomes, and bioinformatics -- An RNA Paradigm -- Epilogue : a few pearls of wisdom.
標題:	RNA - Laboratory manuals. - Analysis -
電子資源:	http://www.sciencedirect.com/science/book/9780122496967
ISBN:	9780122496967

RNA methodologies = a laboratory guide for isolation and characterization /
Farrell, Robert E.{me_controlnum}

RNA methodologiesa laboratory guide for isolation and characterization /[electronic resource] :Robert E. Farrell, Jr. - 3rd ed. - Amsterdam ;Elsevier/Academic Press,c2005. - 1 online resource (xxv, 767 p.) :ill.

Includes bibliographical references and index.

RNA and the cellular biochemistry revisited -- Transcription and the organization of eukaryotic genes -- Messenger RNA -- Resilient ribonucleases -- RNA isolation strategies -- The truth about tissues -- Isolation of polyadenylated RNA -- Quality control for RNA preparations -- Dot blot analysis -- Electrophoresis of RNA -- Photodocumentation and image analysis -- Northern analysis -- Nucleic acid probe technology -- Practical nucleic acid hybridization -- Principles of detection -- Quantification of specific mRNAs by nuclease protection -- Analysis of nuclear RNA -- cDNA synthesis -- RT-PCR -- Quantitative PCR techniques -- Transcript subtraction methods -- mRNA differential display -- High-throughput analysis of gene expression -- RNA interference : targeted gene silencing -- Genomes, transcriptomes, proteomes, and bioinformatics -- An RNA Paradigm -- Epilogue : a few pearls of wisdom.

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5 and 3 RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects.

ISBN: 9780122496967

Source: 98232:98234Elsevier Science & Technologyhttp://www.sciencedirect.comSubjects--Topical Terms:

1362171
RNA
--Analysis--Laboratory manuals.Index Terms--Genre/Form:

542853
Electronic books.

LC Class. No.: QP623 / .F37 2005eb

Dewey Class. No.: 572.8/8

National Library of Medicine Call No.: 2005 F-908

RNA methodologies = a laboratory guide for isolation and characterization /
LDR:03761cmm 2200433Ia 4500 001 1326051
005 20120813090934.0
006 m d
007 cr cn|||||||||
008 130122s2005 ne a ob 001 0 eng d
019 $a 76823712 $a 148737314 $a 517558847 $a 612870061 $a 647545421
020 $a 9780122496967
020 $a 0122496965
020 $a 0080454763 (electronic bk.)
020 $a 9780080454764 (electronic bk.)
029 1 $a NZ1 $b 12434304
029 1 $a YDXCP $b 2493556
029 1 $a AU@ $b 000048129896
029 1 $a DEBBG $b BV039829038
035 $a ocn162129907
037 $a 98232:98234 $b Elsevier Science & Technology $n http://www.sciencedirect.com
040 $a OPELS $b eng $c OPELS $d OKU $d OCLCQ $d N$T $d YDXCP $d E7B $d OCLCQ $d REDDC $d OCLCQ
049 $a NTYA
050 4 $a QP623 $b .F37 2005eb
060 4 $a 2005 F-908
060 4 $a QU 25 $b F245r 2005
072 7 $a SCI $x 049000 $2 bisacsh
082 0 4 $a 572.8/8 $2 22
100 1 $a Farrell, Robert E.{me_controlnum} $3 1362167
245 1 0 $a RNA methodologies $h [electronic resource] : $b a laboratory guide for isolation and characterization / $c Robert E. Farrell, Jr.
250 $a 3rd ed.
260 $a Amsterdam ; $a Boston : $b Elsevier/Academic Press, $c c2005.
300 $a 1 online resource (xxv, 767 p.) : $b ill.
504 $a Includes bibliographical references and index.
505 0 $a RNA and the cellular biochemistry revisited -- Transcription and the organization of eukaryotic genes -- Messenger RNA -- Resilient ribonucleases -- RNA isolation strategies -- The truth about tissues -- Isolation of polyadenylated RNA -- Quality control for RNA preparations -- Dot blot analysis -- Electrophoresis of RNA -- Photodocumentation and image analysis -- Northern analysis -- Nucleic acid probe technology -- Practical nucleic acid hybridization -- Principles of detection -- Quantification of specific mRNAs by nuclease protection -- Analysis of nuclear RNA -- cDNA synthesis -- RT-PCR -- Quantitative PCR techniques -- Transcript subtraction methods -- mRNA differential display -- High-throughput analysis of gene expression -- RNA interference : targeted gene silencing -- Genomes, transcriptomes, proteomes, and bioinformatics -- An RNA Paradigm -- Epilogue : a few pearls of wisdom.
520 $a This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5 and 3 RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects.
588 $a Description based on print version record.
650 0 $a RNA $x Analysis $v Laboratory manuals. $3 1362171
650 1 2 $a RNA $x isolation & purification $v Laboratory Manuals. $3 1362168
650 2 2 $a Molecular Biology $x methods $v Laboratory Manuals. $3 1362169
650 2 2 $a RNA $x physiology $v Laboratory Manuals. $3 1362170
650 6 $a ARN $x Analyse $v Manuels de laboratoire. $3 1615119
650 7 $a SCIENCE $x Life Sciences $x Molecular Biology. $2 bisacsh $3 1362090
650 1 7 $a RNA. $3 569546
650 1 7 $a Laboratoriumonderzoek. $2 gtt $3 958023
655 4 $a Electronic books. $2 lcsh $3 542853
776 0 8 $i Print version: $a Farrell, Robert E. $t RNA methodologies. $b 3rd ed. $d Amsterdam ; Boston : Elsevier/Academic Press, c2005 $z 0122496965 $z 9780122496967 $w (DLC) 2005041012 $w (OCoLC)57414698
856 4 0 $3 ScienceDirect $u http://www.sciencedirect.com/science/book/9780122496967
938 $a YBP Library Services $b YANK $n 2493556
938 $a ebrary $b EBRY $n ebr10138208