| 紀錄類型: |
書目-電子資源
: Monograph/item
|
| 正題名/作者: |
Techniques in protein chemistry VIII/ edited by Daniel R. Marshak.{me_controlnum} |
| 其他題名: |
Techniques in protein chemistry 8 |
| 其他作者: |
Marshak, Daniel R. |
| 出版者: |
San Diego ;Academic Press, : c1997., |
| 面頁冊數: |
1 online resource (xxi, 908 p.) :ill. |
| 附註: |
"Selected from presentations at the Tenth Symposium of the Protein Society, held in San Jose, August 3-7, 1996"--P. xvii. |
| 內容註: |
Foreword. -- Preface. -- Acknowledgments. -- Primary Structural Analysis: Protein Sequencing Using Microreactors and Capillary Electrophoresis with Thermo-optical Absorbance Detection: -- Enhancement of Concentration Limits of Detection in Capillary Electrophoresis: Examples of On-Line Sample Preconcentration, Cleanup, and Microreactor Technology in Protein Characterization. -- Sequencing MHC Class I Peptides Using Membrane Preconcentration-Capillary Electrophoresis Tandem Mass Spectrometry (mPC-CE-MS/MS). -- Nano-electrospray Mass Spectrometry and Edman Sequencing of Peptides and Proteins Collected from Capillary Electrophoresis. -- Characterization of a Recombinant Hepatitis E Protein Vaccine Candidate by Mass Spectrometry and Sequencing Techniques. -- Comparison of the High Sensitivity and Standard Versions of Applied Biosystems Procise TM 494 N-Terminal Protein Sequencers Using Various Sequencing Supports. -- Evaluation of ABRF-96SEQ: A Sequence Assignment Exercise. -- Internal Protein Sequencing of SDS-PAGE-Separated Proteins: Optimization of an In Gel Digest Protocol. -- A Strategy to Obtain Internal Sequence Information from Blotted Proteins after Initial N-terminal Sequencing. -- Internal Protein Sequencing of SDS PAGE-Separated Proteins: A Collaborative ABRF Study. -- Physical and Chemical Analysis: -- Chromatographic Determination of Extinction Coefficients of Non-Glycosylated Proteins Using Refractive Index (RI) and UV Absorbance (UV) Detectors: Applications for Studying Protein Interactions by Size Exclusion Chromatography with Light-Scattering, UV, and RI Detectors. -- Single Alkaline Phosphatase Molecule Assay by Capillary Electrophoresis Laser-Induced Fluorescence Detection. -- A New Centrifugal Device Used in Sample Clean-up and Concentration of Peptides. -- Sample Preparation Using Synthetic Membrances for the Study of Biopolymers by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry. -- Use of LC/MS Peptide Mapping for Characteriation of Isoforms in 15N-Labeled Recombinant Human Leptin. -- Hyphenated HPLC Methodology for the Resolution and Elucidation of Peptides from Proteolytic Digests. -- Detecting and Identifying Active Compounds from a Combinatorial Library Using Iasys and Electrospray Mass Spectrometry. -- Amino Acid Analysis of Unusual and Complex Samples Based on 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatization. -- Development of a Method for Analysis of Free Amino Acids from Physiological Samples Using a 420A ABI/PE Amino Acid Analyzer. -- Quantitation and Identification of Proteins by Amino Acid Analysis: ABRF-96AAA Collaborative Trial -- Chemical Modification: -- Nonaqueous Chemical Modification of Lyophilized Proteins -- Reaction of HIV-1 NC p7 Zinc Fingers with Electrophilic Reagents. -- The Identification and Isolation of Reactive Thiols in Ricin A-Chain and Blocked Ricin Using 2-(4'-Maleimidylanilino)naphthalene-6-sulfonic Acid. -- Inactivation of the Human Cytomegalovirus Protease by Diisopropylfluorophosphate. -- Studies on the Status of Arginine Residues in Phospholipase A<->2 from Naja naja atra (Taiwan cobra) Snake Venom. -- Selective Reduction of the Intermolecular Disulfide Bridge in Human Glial Cell Lined-Derived Neurotrophic Factor Using Tris-(2-Carboxyethyl)Phosphine. -- Effects of Surface Hydrophobicity on the Structural Properties of Insulin. -- The Effects of in Vitro Methionine Oxidation on the Bioactivity and Structure of Human Keratinocyte Growth Factor. -- Posttranslational and Other Modifications: -- Effects of Enzyme Glycosylation on the Chemical Step of Catalysis, as Probed by Hydrogen Tunneling and Enthalpy of Activation. -- Profile Analysis of Oligosaccharides from Glycoproteins by PMP Labeling. -- Comparison of Chemical and Enzymatic Release Methods Using RP-HPLC and Mass Spectrometry. -- Positive Identification of Glycosylation Sites in Proteins and Peptides Using a Modified Beckman LF 3600 N-Terminal Protein Sequencer. -- Deamidation and Isoaspartate Formation during in Vitro Aging of a Recombinant Hepatitis E Vaccine Candidate. -- The Isolation and Characterization of Active Site Peptides in Lysyl Oxidase. -- Complement Activation in EDTA Blood/Plasma Samples May Be Caused by Coagulation Proteases. -- Disulfide-Linked Human Stem Cell Factor Dimer: Method of Identification and Molecular Comparison to the Noncovalent Dimer. -- Autocatalytic Reduction of a Humanized Antibody. -- Interactions of Protein with Ligands: -- Oxygen and Ascorbate Mediated Modification of a Recombinant Hemoglobin. -- Metal Activation and Regulation of E. coli Rnase H. Crystal Structure of Avian Sarcoma Virus Integrase with Bound Essential Cations. -- Multidimensional NMR Studies of an Exchangeable Apolipoprotein and Its Interactions with Lipids. -- NMR Methods for Analysis of CRALBP Retinoid Binding. -- Novel Method for Measuring the Binding Properties of the Site-Directed Mutants of the Proteins that Bind Hydrophobic Ligands: Application to Cellular Retinoic Acid Binding Proteins. -- A Strategy for Predicting the Ligand Binding Competence of Recombinant Orphan Nuclear Receptors Using Biophysical Characterization. -- Protein-Protein Interactions: -- Detection of Intra-Cellular Protein-Protein Intractions: Penicillin Interactive Proteins and Morphogene Proteins. -- Use of Synthetic Peptides in Mapping the Binding Sites for hsp70 in a Mitochondrial Protein. -- Interfacing Biomolecular Interaction Analysis with Mass Spectrometry and the Use of Bioreactive Mass Spectrometer Probe Tips in Protein Characterization. -- Transition-State Theory and Secondary Forces in Antigen-Antibody Complexes. -- Thermodynamic Investigation of Enzyme and Inhibitor Interactions with High Affinity. -- Development and Characterization of a Fab Fragment as a Surrogate for the IL-1 Receptor. -- Macromolecular Assemblies: -- Topogology of Membrane Proteins in Native Membranes Using Matrix-Assisted Laser Desorption Ionization/Mass Spectrometry. -- Role of D-Ser46 in the P-type Calcium Channel Blocker, w-Agatoxin-TK. -- Involvement of Basic Amphiphilic a-Helical Domain in the Reversible Membrane Interaction of Amphitropic Proteins: Structural Studies by Mass Spectrometry, Circular Dichroism, and Nuclear Magnetic Resonance. -- One-Dimensional Diffusion of a Protein along a Single-Stranded Nucleic Acid. -- Metal-Dependent Structure and Self Association of the RAG1 Zinc-Binding Domain. -- Localizing Flexibility within the Target Site of DNA-Bending Proteins. -- Assembly of the Multifunctional EcoKI DNA Restriction Enzyme in Vitro. -- Three Dimensional Structure: -- Strategies for NMR Assignment and Global Fold Determinations Using Perdeuterated Proteins. -- 1H-NMR Evidence for Two Buried ASN Side-Chains in the c-MYC-MAX Heterodimeric a-Helical Coiled-Coil. -- NMR Confirms the Presence of the Amino-Terminal Helix of Group II Phospholipase A<->2 in Solution. -- The Crystallographic Analysis of Glycosylation-Inhibiting Factor -- Structure of the D30N Active Site Mutant of FIV Proteinase Complexed with a Statine-Based Inhibitor. -- A Homology-Based Model of Juvenile Hormone Esterase from the Crop Pest, Heliothis virescens. -- Analysis of Linkers of Regular Secondary Structures in Proteins. -- Structural and Functiona Roles of Tyrosine-50 of Yeast Guanylate Kinase. -- Dynamics and Folding: -- Flexibility of Serine Protease in Nonaqueous Solvent. -- Internal Dynamics of Human Ubiquitin Revealed by 13C-Relaxation Studies of Randomly Fractionally Labeled Protein. -- Detection of Protein Unfolding and Fluctuations by Native State Hydrogen Exchange. -- Laser Temperature Jump for the Study of Early Events in Protein Folding. -- Biophysical and Structural Analysis of Human Acidic Fibroblast Growth Factor. -- A Thermodynamic Analysis Discriminating Loop Backbone. -- The Equilibrium Ensemble of Conformational States in Staphylococcal Nuclease. -- An Evaluation of Protein Secondary Structure Prediction Algorithms. -- Biological and Chemical Design: -- Designing Water Soluble b-Sheet Peptides with Compact Structure. -- Engineering Secondary Structure to Invert Coenzyme Specificity in Isopropylmalate Dehydrogenase. -- A Method for Determining Domain Binding Sites in Proteins with Swapped Domains: Implications for bA3- and bB2- Crystallins. -- Complete Mutagenesis of the Gene Encoding TEM-1 b-Lactamase. -- Characterization of Truncated Kirsten-Ras Purified from Baculovirus Infected Insect Cells Indicates Heterogeneity due to N-terminal Processing and Nucleotide Dissociation. -- Isolation and Characterization of Multiple-Methionine Mutants of T4 Lysozyme with Simplified Cores. -- Synthesis of Alzheimer's (1-42) Ab-Amyloid Peptide with Preformed Fmoc-Aminoacyl Fluorides. -- Analysis of Racemization during "Standard" Solid Phase Peptide Synthesis: A Multicenter Study. -- Index. |
| 標題: |
Proteins - Handbooks, manuals, etc. - Analysis - |
| 電子資源: |
http://www.sciencedirect.com/science/bookseries/10808914 |
| ISBN: |
9780124735576 |