東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

FindBook

Google Book

Amazon

博客來

G-Protein and Protein Kinase a Signaling Networks Control Contractility and Calcium Signaling in the Caenorhabditis elegans Spermatheca.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	G-Protein and Protein Kinase a Signaling Networks Control Contractility and Calcium Signaling in the Caenorhabditis elegans Spermatheca./
作者:	Castaneda, Perla G.
出版者:	Ann Arbor : ProQuest Dissertations & Theses, : 2021,
面頁冊數:	130 p.
附註:	Source: Dissertations Abstracts International, Volume: 83-03, Section: B.
Contained By:	Dissertations Abstracts International83-03B.
標題:	Biology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28717525
ISBN:	9798538124725

G-Protein and Protein Kinase a Signaling Networks Control Contractility and Calcium Signaling in the Caenorhabditis elegans Spermatheca.
Castaneda, Perla G.

G-Protein and Protein Kinase a Signaling Networks Control Contractility and Calcium Signaling in the Caenorhabditis elegans Spermatheca. - Ann Arbor : ProQuest Dissertations & Theses, 2021 - 130 p.

Source: Dissertations Abstracts International, Volume: 83-03, Section: B.

Thesis (Ph.D.)--Northeastern University, 2021.

This item must not be sold to any third party vendors.

Correct regulation of contractility and dilation in biological tubes is critical for the function of the reproductive, cardiovascular, and other systems. Dysregulation of the signaling networks that control actomyosin contractility can result in diseases such as cardiovascular diseases and asthma. The reproductive system of the hermaphroditic nematode C. elegans contains a contractile tube of myoepithelial cells known as the spermatheca. The spermatheca stores sperm and is the site of oocyte fertilization. Regulated contraction of the spermatheca pushes the embryo into the uterus. These contractions are dependent on actin and myosin and are in part regulated by Ca2+ signaling through the phospholipase PLC-1, which mediates Ca2+ release from the ER.Here I describe the roles of many genes in the regulation of calcium release and contractility in the C. elegans spermatheca, including the G-protein alpha subunit GSA-1/Gαs, and its downstream effectors, the catalytic subunit KIN-1/PKA-C and the regulatory subunit KIN-2/PKA-R of protein kinase A, and G-protein alpha subunit GOA-1/Gαo. I discuss regulators of this pathway, including the phosphodiesterase PDE-6, the phospholipase PLC-1, and possible upstream regulators such as G-protein coupled receptors (GPCR), the G-protein regulators GPR-1 and GPR-2, and the scaffolding protein ERM-1.Without GSA-1/ Gαs or KIN-1/PKA-C, calcium is not released, and oocytes become trapped in the spermatheca. Conversely, when PKA is activated through a gain of function allele in GSA-1 (GSA-1(GF)) or by depletion of KIN-2/PKA-R, Ca2+ is increased, and waves of Ca2+ travel across the spermatheca even in the absence of oocyte entry. In the sp-ut valve, loss of GSA-1/ Gαs or KIN-1/PKA-C results in sustained, high levels of Ca2+ and a loss of coordination between the spermathecal bag and sp-ut valve. Additionally, I show that depleting phosphodiesterase PDE-6 levels alters contractility and Ca2+ dynamics in the spermatheca, and that GPB-1 and GPB-2, Gβ subunits, play a central role in regulating spermathecal contractility and Ca2+ signaling. This work identifies a signaling network in which Ca2+ and cAMP pathways work together to provide the correct coordinated contractions needed by the spermatheca for a successful ovulation.

ISBN: 9798538124725Subjects--Topical Terms:

522710
Biology.
Subjects--Index Terms:

Actomyosin contractility

G-Protein and Protein Kinase a Signaling Networks Control Contractility and Calcium Signaling in the Caenorhabditis elegans Spermatheca.
LDR:03569nmm a2200397 4500 001 2351935
005 20221111113717.5
008 241004s2021 ||||||||||||||||| ||eng d
020 $a 9798538124725
035 $a (MiAaPQ)AAI28717525
035 $a AAI28717525
040 $a MiAaPQ $c MiAaPQ
100 1 $a Castaneda, Perla G. $3 3691530
245 1 0 $a G-Protein and Protein Kinase a Signaling Networks Control Contractility and Calcium Signaling in the Caenorhabditis elegans Spermatheca.
260 1 $a Ann Arbor : $b ProQuest Dissertations & Theses, $c 2021
300 $a 130 p.
500 $a Source: Dissertations Abstracts International, Volume: 83-03, Section: B.
500 $a Advisor: Cram, Erin J.
502 $a Thesis (Ph.D.)--Northeastern University, 2021.
506 $a This item must not be sold to any third party vendors.
520 $a Correct regulation of contractility and dilation in biological tubes is critical for the function of the reproductive, cardiovascular, and other systems. Dysregulation of the signaling networks that control actomyosin contractility can result in diseases such as cardiovascular diseases and asthma. The reproductive system of the hermaphroditic nematode C. elegans contains a contractile tube of myoepithelial cells known as the spermatheca. The spermatheca stores sperm and is the site of oocyte fertilization. Regulated contraction of the spermatheca pushes the embryo into the uterus. These contractions are dependent on actin and myosin and are in part regulated by Ca2+ signaling through the phospholipase PLC-1, which mediates Ca2+ release from the ER.Here I describe the roles of many genes in the regulation of calcium release and contractility in the C. elegans spermatheca, including the G-protein alpha subunit GSA-1/Gαs, and its downstream effectors, the catalytic subunit KIN-1/PKA-C and the regulatory subunit KIN-2/PKA-R of protein kinase A, and G-protein alpha subunit GOA-1/Gαo. I discuss regulators of this pathway, including the phosphodiesterase PDE-6, the phospholipase PLC-1, and possible upstream regulators such as G-protein coupled receptors (GPCR), the G-protein regulators GPR-1 and GPR-2, and the scaffolding protein ERM-1.Without GSA-1/ Gαs or KIN-1/PKA-C, calcium is not released, and oocytes become trapped in the spermatheca. Conversely, when PKA is activated through a gain of function allele in GSA-1 (GSA-1(GF)) or by depletion of KIN-2/PKA-R, Ca2+ is increased, and waves of Ca2+ travel across the spermatheca even in the absence of oocyte entry. In the sp-ut valve, loss of GSA-1/ Gαs or KIN-1/PKA-C results in sustained, high levels of Ca2+ and a loss of coordination between the spermathecal bag and sp-ut valve. Additionally, I show that depleting phosphodiesterase PDE-6 levels alters contractility and Ca2+ dynamics in the spermatheca, and that GPB-1 and GPB-2, Gβ subunits, play a central role in regulating spermathecal contractility and Ca2+ signaling. This work identifies a signaling network in which Ca2+ and cAMP pathways work together to provide the correct coordinated contractions needed by the spermatheca for a successful ovulation.
590 $a School code: 0160.
650 4 $a Biology. $3 522710
650 4 $a Ovulation. $3 3691531
650 4 $a Endoplasmic reticulum. $3 663051
650 4 $a Reproductive system. $3 3691532
650 4 $a Binding sites. $3 3560349
650 4 $a Metabolism. $3 541349
650 4 $a Peptides. $3 605772
650 4 $a Localization. $3 3560711
650 4 $a Genotype & phenotype. $3 3561790
650 4 $a Genes. $3 600676
650 4 $a Kinases. $3 3558077
650 4 $a Nematodes. $3 3561776
650 4 $a Smooth muscle. $3 662085
650 4 $a Proteins. $3 558769
650 4 $a Embryos. $3 2152917
650 4 $a Neurons. $3 588699
650 4 $a Mammals. $3 531989
650 4 $a Regulation. $3 3561775
650 4 $a Gases. $3 559387
650 4 $a Cell adhesion & migration. $3 3561734
650 4 $a Adenosine triphosphate. $3 3331834
650 4 $a Sensors. $3 3549539
650 4 $a Sperm. $3 3689357
650 4 $a Ligands. $3 686413
650 4 $a Worms. $3 3561322
650 4 $a Phosphorylation. $3 816803
650 4 $a Molecular biology. $3 517296
650 4 $a Biochemistry. $3 518028
650 4 $a Biomechanics. $3 548685
653 $a Actomyosin contractility
653 $a Regulated contraction
653 $a Signaling pathways
653 $a Calcium release regulation
653 $a Ca2+
653 $a cAMP
690 $a 0306
690 $a 0487
690 $a 0648
690 $a 0307
710 2 $a Northeastern University. $b Biology. $3 2091875
773 0 $t Dissertations Abstracts International $g 83-03B.
790 $a 0160
791 $a Ph.D.
792 $a 2021
793 $a English
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=28717525