| Record Type: |
Electronic resources
: Monograph/item
|
| Title/Author: |
Protein phosphorylation/ edited by D.G. Hardie. |
| Reminder of title: |
a practical approach / |
| other author: |
Hardie, D. G. |
| Published: |
London ;Oxford University Press, : 1999., |
| Description: |
xxviii, 431 p. :ill. ;24 cm. |
| Series: |
The practical approach series ; |
| [NT 15003449]: |
Study of protein phosphorylation in intact cells -- Analysis of signal transduction pathways using protein kinase inhibitors and activators -- Analysis of signal transduction pathways using protein-serin/threonine phosphatase inhibitors -- Analysis of signal transduction pathways using molecular biological manipulations -- Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates -- Phosphorylation site analysis by mass spectrometry -- Assay and purification of protein (serine/threonine) phosphatases -- Identification of substrates for protein-tyrosine phosphateases -- Assay and purification of protein-serine/threonine kinases -- Assay of protein kinases and phosphatases using specific peptide substrates -- Cloning and expression of cDNAs encoding protein kinase subunits -- Interaction cloning of protein kinase partners -- Identifying protein kinase substrates by expression screening with solid-phase phosphorylation -- Analysis of protein kinase interactions using the two-hybrid method -- Analysis of protein kinase interactions using biomolecular interaction analysis -- Analysis of protein kinase specificity using oriented peptide libraries -- Crystallization of protein kinases and phosphatases. |
| Subject: |
Phosphoprotein phosphatases - Laboratory manuals. - |
| Online resource: |
https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98466An electronic book accessible through the World Wide Web; click for information |
| ISBN: |
0585483930 (electronic bk.) |