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Cryo-electron tomography = structural biology in situ /

Record Type:	Electronic resources : Monograph/item
Title/Author:	Cryo-electron tomography/ edited by Friedrich Forster, Ariane Briegel.
Reminder of title:	structural biology in situ /
other author:	Forster, Friedrich.
Published:	Cham :Springer International Publishing : : 2024.,
Description:	viii, 389 p. :ill. (chiefly col.), digital ;24 cm.
[NT 15003449]:	1. Fundamentals of Instrumentation and electron optics For cryo-electron tomography -- 2. Principles of tomographic reconstruction -- 3. Cryo-preparation for cellular tomography -- 4. Cryo-FIB milling -- 5. In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques -- 6. Labeling approaches and correlation with light microscopy (Cryo) CLEM -- 7. Data collection -- 8. Subvolume averaging and classification -- 9. High resolution tomography/ Molecular model building and visualization -- 10. Beam-induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography -- 11. Tomogram segmentation -- 12. Electron tomography of cryo-fixed and resin-embedded samples -- 13. Democratization of tomographic data / FAIR data.
Contained By:	Springer Nature eBook
Subject:	Tomography. -
Online resource:	https://doi.org/10.1007/978-3-031-51171-4
ISBN:	9783031511714

Cryo-electron tomography = structural biology in situ /
Cryo-electron tomographystructural biology in situ /[electronic resource] :edited by Friedrich Forster, Ariane Briegel. - Cham :Springer International Publishing :2024. - viii, 389 p. :ill. (chiefly col.), digital ;24 cm. - Focus on structural biology,v. 112542-9566 ;. - Focus on structural biology ;v. 11..

1. Fundamentals of Instrumentation and electron optics For cryo-electron tomography -- 2. Principles of tomographic reconstruction -- 3. Cryo-preparation for cellular tomography -- 4. Cryo-FIB milling -- 5. In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques -- 6. Labeling approaches and correlation with light microscopy (Cryo) CLEM -- 7. Data collection -- 8. Subvolume averaging and classification -- 9. High resolution tomography/ Molecular model building and visualization -- 10. Beam-induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography -- 11. Tomogram segmentation -- 12. Electron tomography of cryo-fixed and resin-embedded samples -- 13. Democratization of tomographic data / FAIR data.

This book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses. This book provides a unique reference for the emerging field of cryo-ET. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis, and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, and molecular biology and highly valuable for the more experienced and specialized PhD student. Furthermore, it stands as a state-of-the-art source of knowledge for the established senior scientist within the field of structural biology.

ISBN: 9783031511714

Standard No.: 10.1007/978-3-031-51171-4doiSubjects--Topical Terms:

836553
Tomography.

LC Class. No.: RC78.7.T6 / C97 2024

Dewey Class. No.: 616.075722

Cryo-electron tomography = structural biology in situ /
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260 $a Cham : $b Springer International Publishing : $b Imprint: Springer, $c 2024.
300 $a viii, 389 p. : $b ill. (chiefly col.), digital ; $c 24 cm.
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505 0 $a 1. Fundamentals of Instrumentation and electron optics For cryo-electron tomography -- 2. Principles of tomographic reconstruction -- 3. Cryo-preparation for cellular tomography -- 4. Cryo-FIB milling -- 5. In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques -- 6. Labeling approaches and correlation with light microscopy (Cryo) CLEM -- 7. Data collection -- 8. Subvolume averaging and classification -- 9. High resolution tomography/ Molecular model building and visualization -- 10. Beam-induced Motion Mechanism and Correction for Improved Cryo-Electron Microscopy and Cryo-Electron Tomography -- 11. Tomogram segmentation -- 12. Electron tomography of cryo-fixed and resin-embedded samples -- 13. Democratization of tomographic data / FAIR data.
520 $a This book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses. This book provides a unique reference for the emerging field of cryo-ET. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis, and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, and molecular biology and highly valuable for the more experienced and specialized PhD student. Furthermore, it stands as a state-of-the-art source of knowledge for the established senior scientist within the field of structural biology.
650 0 $a Tomography. $3 836553
650 0 $a Imaging systems in biology. $3 629357
650 0 $a Cryopreservation of organs, tissues, etc. $3 663579
650 1 4 $a Structural Biology. $3 3538470
650 2 4 $a Biophysical Methods. $3 3634701
650 2 4 $a Immunology. $3 611031
650 2 4 $a Microscopy. $3 540544
700 1 $a Forster, Friedrich. $3 3714270
700 1 $a Briegel, Ariane. $3 3714271
710 2 $a SpringerLink (Online service) $3 836513
773 0 $t Springer Nature eBook
830 0 $a Focus on structural biology ; $v v. 11. $3 3714272
856 4 0 $u https://doi.org/10.1007/978-3-031-51171-4
950 $a Biomedical and Life Sciences (SpringerNature-11642)